Department of Microbiology and Hospital Hygiene, Bacterial Virulence and Chronic Infections, INSERM U1047, CHU Nîmes Univiversity Montpellier, CEDEX 09, 30029 Nîmes, France.
Department of Infectious and Tropical Diseases, CH Perpignan, 66000 Perpignan, France.
Int J Mol Sci. 2023 Jan 12;24(2):1547. doi: 10.3390/ijms24021547.
Coagulase-negative staphylococci (CoNS) and especially are responsible for health care infections, notably in the presence of foreign material (e.g., venous or central-line catheters). Catheter-related bacteremia (CRB) increases health care costs and mortality. The aim of our study was to evaluate the impact of 15 days of antibiotic exposure (ceftobiprole, daptomycin, linezolid and vancomycin) at sub-inhibitory concentration on the resistance, fitness and genome evolution of 36 clinical strains of responsible for CRB. Resistance was evaluated by antibiogram, the ability to adapt metabolism by the Biofilm Ring test and the in vivo nematode virulence model. The impact of antibiotic exposure was determined by whole-genome sequencing (WGS) and biofilm formation experiments. We observed that strains presented a wide variety of virulence potential and biofilm formation. After antibiotic exposure, strains adapted their fitness with an increase in biofilm formation. Antibiotic exposure also affected genes involved in resistance and was responsible for cross-resistance between vancomycin, daptomycin and ceftobiprole. Our data confirmed that antibiotic exposure modified bacterial pathogenicity and the emergence of resistant bacteria.
凝固酶阴性葡萄球菌（CoNS），特别是 ，是导致医疗保健感染的原因，特别是在存在异物（例如，静脉或中央导管）的情况下。导管相关性菌血症（CRB）会增加医疗保健成本和死亡率。我们的研究旨在评估亚抑菌浓度下 15 天的抗生素暴露（头孢洛林、达托霉素、利奈唑胺和万古霉素）对 36 株导致 CRB 的 临床菌株的耐药性、适应性和基因组进化的影响。通过抗生素图谱评估耐药性，通过生物膜环试验评估代谢适应能力，通过体内线虫毒力模型评估毒力。通过全基因组测序（WGS）和生物膜形成实验确定抗生素暴露的影响。我们观察到 菌株表现出广泛的毒力潜力和生物膜形成能力。抗生素暴露后， 菌株通过增加生物膜形成来适应其适应性。抗生素暴露还影响了耐药性相关基因，并导致万古霉素、达托霉素和头孢洛林之间的交叉耐药性。我们的数据证实，抗生素暴露改变了细菌的致病性和耐药菌的出现。
