Song Jiehui, Lei Tengyu, Hao Xiaojuan, Yuan Huizhu, Sun Wei, Chen Shuning
Key Laboratory of Pesticides Evaluation, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Jiangsu Key Laboratory of Crop Genetics and Physiology & Co-Innovation Center for Modern Production Technology of Grain Crops, Agricultural College, Yangzhou University, Yangzhou 225009, China.
Microorganisms. 2022 Dec 21;11(1):20. doi: 10.3390/microorganisms11010020.
Gray mold caused by is a devastating disease in tomatoes. Site-specific fungicide application is still key to disease management; however, chemical control has many drawbacks. Here, the combined application of a biological agent, , with newly developed succinate dehydrogenase inhibitors (SDHI) fungicides showed stronger synergistic effects than the application of SDHI fungicides alone on tomato gray mold control. 67-1 has been reported as an efficient biological control agent (BCA) for . Little information is currently available about the combination of and fungicides in the control of gray mold. By testing the sensitivity to fungicides with different action mechanisms, isolates showed high tolerance to SDHI fungicides (1000 μg mL) on PDA, and the conidial germination rate was almost not affected under 120 μg mL of fluxapyroxad and fluopyram. In greenhouse experiments, the control effect of the combination of and fluxapyroxad or fluopyram against tomato gray mold was significantly increased than the application of BCA or SDHI fungicides alone, and the combination allows a two-fold reduction of both the fungicide and BCA dose. Further, the biomass of and on tomato plants was determined by qPCR. For , the trend of detection level for different treatments was consistent with that of the pot experiments, and the lowest biomass of was found when treated with combined with fluxapyroxad and fluopyram, respectively. For , qPCR assay confirmed its colonization on tomato plants when mixed with fluopyram and fluxapyroxad. These results indicated that combining 67-1 with the SDHI fungicides could synergistically increase control efficacy against tomato gray mold.
由[未提及具体病原菌名称]引起的灰霉病是番茄的一种毁灭性病害。针对特定部位施用杀菌剂仍是病害管理的关键；然而，化学防治存在诸多弊端。在此，一种生物制剂[未提及具体生物制剂名称]与新开发的琥珀酸脱氢酶抑制剂（SDHI）类杀菌剂联合施用，在防治番茄灰霉病方面比单独施用SDHI类杀菌剂表现出更强的协同效应。67 - 1已被报道为[未提及具体防治对象]的一种高效生物防治剂（BCA）。目前关于[未提及具体生物制剂名称]与杀菌剂联合防治灰霉病的信息较少。通过测试对不同作用机制杀菌剂的敏感性，[未提及具体病原菌名称]分离株在PDA上对SDHI类杀菌剂（1000μg/mL）表现出高耐受性，在120μg/mL的氟唑菌酰胺和吡唑萘菌胺处理下分生孢子萌发率几乎不受影响。在温室试验中，[未提及具体生物制剂名称]与氟唑菌酰胺或吡唑萘菌胺联合施用对番茄灰霉病的防治效果比单独施用生物防治剂或SDHI类杀菌剂显著提高，且联合施用可使杀菌剂和生物防治剂的用量均减少一半。此外，通过qPCR测定了[未提及具体生物制剂名称]和[未提及具体病原菌名称]在番茄植株上的生物量。对于[未提及具体生物制剂名称]，不同处理的检测水平趋势与盆栽试验一致，分别与氟唑菌酰胺和吡唑萘菌胺联合处理时[未提及具体生物制剂名称]的生物量最低。对于[未提及具体病原菌名称]，qPCR检测证实其与吡唑萘菌胺和氟唑菌酰胺混合时可在番茄植株上定殖。这些结果表明，将67 - 1与SDHI类杀菌剂联合使用可协同提高对番茄灰霉病的防治效果。
