Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China.
Institute of Basic Medical Sciences, Khyber Medical University, Peshawar 25100, Pakistan.
Molecules. 2023 Jan 4;28(2):491. doi: 10.3390/molecules28020491.
All nutrient-rich feed and food environments, as well as animal and human mucosae, include lactic acid bacteria known as Lactobacillus plantarum. This study reveals an advanced analysis to study the interaction of probiotics with the gastrointestinal environment, irritable bowel disease, and immune responses along with the analysis of the secondary metabolites’ characteristics of Lp YW11. Whole genome sequencing of Lp YW11 revealed 2297 genes and 1078 functional categories of which 223 relate to carbohydrate metabolism, 21 against stress response, and the remaining 834 are involved in different cellular and metabolic pathways. Moreover, it was found that Lp YW11 consists of carbohydrate-active enzymes, which mainly contribute to 37 glycoside hydrolase and 28 glycosyltransferase enzyme coding genes. The probiotics obtained from the BACTIBASE database (streptin and Ruminococcin-A bacteriocins) were docked with virulent proteins (cdt, spvB, stxB, and ymt) of Salmonella, Shigella, Campylobacter, and Yersinia, respectively. These bacteria are the main pathogenic gut microbes that play a key role in causing various gastrointestinal diseases. The molecular docking, dynamics, and immune simulation analysis in this study predicted streptin and Ruminococcin-A as potent nutritive bacteriocins against gut symbiotic pathogens.
所有富含营养的饲料和食物环境，以及动物和人类的黏膜，都包含被称为植物乳杆菌的乳酸菌。本研究揭示了一种先进的分析方法，用于研究益生菌与胃肠道环境、肠易激综合征和免疫反应的相互作用，以及分析 Lp YW11 的次生代谢产物特征。Lp YW11 的全基因组测序揭示了 2297 个基因和 1078 个功能类别，其中 223 个与碳水化合物代谢有关，21 个与应激反应有关，其余 834 个涉及不同的细胞和代谢途径。此外，研究发现 Lp YW11 包含碳水化合物活性酶，主要涉及 37 种糖苷水解酶和 28 种糖基转移酶编码基因。从 BACTIBASE 数据库（链霉素和 Ruminococcin-A 细菌素）获得的益生菌分别与沙门氏菌、志贺氏菌、弯曲杆菌和耶尔森氏菌的毒力蛋白（cdt、spvB、stx B 和 ymt）对接。这些细菌是主要的肠道致病菌，在引起各种胃肠道疾病方面起着关键作用。本研究中的分子对接、动力学和免疫模拟分析预测链霉素和 Ruminococcin-A 是针对肠道共生病原体的有效营养细菌素。
