Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 21955, Saudi Arabia.
Laboratoires TBC, Faculty of Pharmaceutical and Biological Sciences, B.P. 8359006 Lille, France.
Molecules. 2023 Jan 6;28(2):614. doi: 10.3390/molecules28020614.
This study aimed to determine the chemical composition of the essential oils (EOs) of L., as well as to evaluate the antibacterial, antidiabetic, dermatoprotective, and anti-inflammatory properties, and the EOs and aqueous extracts of . The antibacterial activity was evaluated against bacterial strains, Gram-positive and Gram-negative, using the well diffusion and microdilution methods, whereas the antidiabetic activity was assessed in vitro using two enzymes involved in carbohydrate digestion, α-amylase and α-glucosidase. On the other hand, the dermatoprotective and anti-inflammatory activities were studied by testing tyrosinase and lipoxygenase inhibition activity, respectively. The results showed that the chemical composition of EO (OBEO) is dominated by methyl chavicol (86%) and -anethol (8%). OBEO exhibited significant antibacterial effects against Gram-negative and Gram-positive strains, demonstrated by considerable diameters of the inhibition zones and lower MIC and MBC values. In addition, OBEO exhibited significant inhibition of α-amylase (IC = 50.51 ± 0.32 μg/mL) and α-glucosidase (IC = 39.84 ± 1.2 μg/mL). Concerning the anti-inflammatory activity, OBEO significantly inhibited lipoxygenase activity (IC = 18.28 ± 0.03 μg/mL) compared to the aqueous extract (IC = 24.8 ± 0.01 μg/mL). Moreover, tyrosinase was considerably inhibited by OBEO (IC = 68.58 ± 0.03 μg/mL) compared to the aqueous extract (IC = 118.37 ± 0.05 μg/mL). The toxicological investigations revealed the safety of in acute and chronic toxicity. The finding of in silico analysis showed that methyl chavicol and -anethole (main compounds of OBEO) validate the pharmacokinetics of these compounds and decipher some antibacterial targets.
本研究旨在确定 L. 的精油（EOs）的化学成分，并评估其抗菌、抗糖尿病、皮肤保护和抗炎特性，以及 和水提物的抗菌、抗糖尿病、皮肤保护和抗炎特性。使用平板扩散法和微量稀释法评估抗菌活性，针对革兰氏阳性和革兰氏阴性细菌菌株；体外评估抗糖尿病活性，使用两种参与碳水化合物消化的酶，α-淀粉酶和α-葡萄糖苷酶。另一方面，通过测试酪氨酸酶和脂氧合酶抑制活性来研究皮肤保护和抗炎活性。结果表明， EO（OBEO）的化学成分以甲基丁香酚（86%）和 -茴香脑（8%）为主。OBEO 对革兰氏阴性和革兰氏阳性菌株表现出显著的抗菌作用，表现为抑制带的直径相当大，MIC 和 MBC 值较低。此外，OBEO 对 α-淀粉酶（IC = 50.51 ± 0.32 μg/mL）和 α-葡萄糖苷酶（IC = 39.84 ± 1.2 μg/mL）表现出显著的抑制作用。关于抗炎活性，与水提物（IC = 24.8 ± 0.01 μg/mL）相比，OBEO 显著抑制脂氧合酶活性（IC = 18.28 ± 0.03 μg/mL）。此外，与水提物（IC = 118.37 ± 0.05 μg/mL）相比，OBEO 对酪氨酸酶的抑制作用相当显著（IC = 68.58 ± 0.03 μg/mL）。急性和慢性毒性毒理学研究表明 的安全性。计算机分析的结果表明，甲基丁香酚和 -茴香脑（OBEO 的主要化合物）验证了这些化合物的药代动力学，并解析了一些抗菌靶点。
