and Evaluation for the Inhibitory Action of Methanol Extract on -Glucosidase and -Amylase.

Diabetes mellitus is a metabolic disease that predominates, nowadays. It causes hyperglycemia and consequently major health complications. Type II diabetes is the most common form and is a result of insulin resistance in the target tissues. To treat this disease, several mechanisms have been proposed. The most direct route is via inhibiting the intestinal enzymes, e.g., -glucosidase and -amylase, responsible for intestinal polysaccharide digestion that therefore would reduce the absorption of monosugars through the intestinal walls. In this study, we shed the light on this route by testing the inhibitory effect of extract on the enzymes -glucosidase and -amylase and . Experimental procedures were performed to test the effect of the methanol extract from aerial parts followed by the docking. 500 g/mL of the extract led to 70.2% ± 8.6 and 25.4% ± 3.3 inhibition on -glucosidase and -amylase activity, respectively. Similarly, the effect of caffeic acid, a major extract ingredient, was also tested, and it caused 42.7% ± 3.0 and 47.1% ± 4.0 inhibition for -amylase and -glucosidase, respectively. Docking experiments were performed to predict the phytochemicals responsible for this robust inhibitory activity in the extracts. Several compounds have shown variable levels of inhibition, e.g., caffeic acid, pyroglutamic acid, and uvasol. The results indicated that can be a potent antidiabetic drug.