School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso 2340000, Chile.
Laboratorio de Tecnologia Enzimatica para Bioprocesos, Instituto de Investigación Multidisciplinario en Ciencia y Tecnología, Universidad de La Serena, Raúl Bitran 1305, La Serena 1700000, Chile.
Molecules. 2023 Jan 8;28(2):644. doi: 10.3390/molecules28020644.
Ascorbyl palmitate, an ascorbic acid ester, is an important amphipathic antioxidant that has several applications in foods, pharmaceuticals, and cosmetics. The enzymatic synthesis of ascorbyl palmitate is very attractive, but few efforts have been made to address its process scale-up and implementation. This study aimed at evaluating the enzymatic synthesis of ascorbyl palmitate in a rotating basket reactor operated in sequential batches. Different commercial immobilized lipases were tested, and the most suitable reaction conditions were established. Among those lipases studied were Amano Lipase PS, Lipozyme TL IM, Lipozyme Novo 40086, Lipozyme RM IM and Lipozyme 435. Initially, the enzymes were screened based on previously defined synthesis conditions, showing clear differences in behavior. Lipozyme 435 proved to be the best catalyst, reaching the highest values of initial reaction rate and yield. Therefore, it was selected for the following studies. Among the solvents assayed, 2-methyl-2-butanol and acetone showed the highest yields, but the operational stability of the catalyst was better in 2-methyl-2-butanol. The tests in a basket reactor showed great potential for large-scale application. Yields remained over 80% after four sequential batches, and the basket allowed for easy catalyst recycling. The results obtained in basket reactor are certainly a contribution to the enzymatic synthesis of ascorbyl palmitate as a competitive alternative to chemical synthesis. This may inspire future cost-effectiveness studies of the process to assess its potential as a viable alternative to be implemented.
抗坏血酸棕榈酸酯是一种抗坏血酸酯，是一种重要的两亲性抗氧化剂，在食品、制药和化妆品中有多种应用。抗坏血酸棕榈酸酯的酶合成非常有吸引力，但很少有人努力解决其工艺放大和实施的问题。本研究旨在评估在连续分批操作的旋转篮式反应器中酶法合成抗坏血酸棕榈酸酯。测试了不同的商业固定化脂肪酶，并确定了最合适的反应条件。在研究的脂肪酶中，有 Amano Lipase PS、Lipozyme TL IM、Lipozyme Novo 40086、Lipozyme RM IM 和 Lipozyme 435。最初，根据之前定义的合成条件筛选酶，显示出明显的行为差异。Lipozyme 435 被证明是最好的催化剂，达到了最高的初始反应速率和产率。因此，它被选用于后续研究。在所测试的溶剂中，2-甲基-2-丁醇和丙酮显示出最高的产率，但在 2-甲基-2-丁醇中催化剂的操作稳定性更好。篮式反应器中的测试显示出大规模应用的巨大潜力。经过四个连续批次后，产率仍保持在 80%以上，篮式反应器便于催化剂的回收。在篮式反应器中获得的结果无疑对抗坏血酸棕榈酸酯的酶合成是一种有竞争力的替代化学合成的贡献。这可能会激发未来对该工艺的成本效益研究，以评估其作为可行替代方案实施的潜力。
