Laboratory of Analytical Chemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Laboratory of Pharmaceutical Analysis, Department of Pharmaceutical Technology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Molecules. 2023 Jan 16;28(2):888. doi: 10.3390/molecules28020888.
In this communication, we describe the first analytical method for the determination of free histidine in hair care products (shampoos and conditioners). Cation-exchange chromatography combined with postcolumn derivatization and fluorimetric detection enabled the accurate (recovery: 83.5-114.8%) and precise (2.4-5.6% RSD) determination of free histidine without matrix interferences at concentration levels down to 1.5 mg kg. Real commercially available samples were found to contain the amino acid at levels ranging between 70 and 535 mg kg.
在本通讯中，我们描述了一种用于测定护发产品（洗发水和护发素）中游离组氨酸的分析方法。阳离子交换色谱结合柱后衍生化和荧光检测，可在 1.5mg/kg 浓度水平下实现游离组氨酸的准确（回收率：83.5-114.8%）和精密度（RSD：2.4-5.6%）测定，且不受基质干扰。发现实际市售样品中含有该氨基酸，含量在 70-535mg/kg 之间。
