Ngobeni Renay, Ramalivhana Jeffrey Naledzani, Traore Afsatou Ndama, Samie Amidou
Department of Biochemistry and Microbiology, University of Venda, Thohoyandou 0950, Limpopo, South Africa.
Department of Medicine, Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
Pathogens. 2022 Dec 23;12(1):19. doi: 10.3390/pathogens12010019.
Infections by the parasite are increasing in HIV-infected individuals. Interleukin (IL-10) plays an important role in maintaining the mucosal barrier. Therefore, the seroprevalence of was investigated in relation to the IL-10 serum concentration among HIV- infected patients. A total of 647 blood samples were collected from asymptomatic HIV-infected patients. The antigen (GALNAC lectin) and serum antibodies were assessed using specific ELISAs (TECHLAB, Virginia, USA). IL10 blood levels were measured using a commercial ELISA test, and the results were analyzed using parametric and non-parametric statistical tests. The Gal/GALNAC lectin was detected in only 0.5% (3/647) of individuals, and the antibodies against were detected in 65.2% (422/647) of the samples. A significant increase in IL-10 levels was found in 68.1% of patients who were sero-negative for antibodies compared to patients who were sero-positive. There is a high level of exposure to among HIV patients in South Africa, although the prevalence of amoebic liver abscesses might be low. This study revealed that elevated levels of IL-10 might be associated with a reduced risk of amebiasis.
在感染HIV的个体中，该寄生虫感染正在增加。白细胞介素（IL-10）在维持黏膜屏障方面发挥着重要作用。因此，针对HIV感染患者，研究了该寄生虫的血清阳性率与IL-10血清浓度之间的关系。总共从无症状HIV感染患者中采集了647份血样。使用特定的酶联免疫吸附测定法（ELISA，美国弗吉尼亚州TECHLAB公司）评估该寄生虫抗原（GALNAC凝集素）和血清抗体。使用商用ELISA检测法测量IL-10血液水平，并使用参数和非参数统计检验分析结果。仅在0.5%（3/647）的个体中检测到Gal/GALNAC凝集素，在65.2%（422/647）的样本中检测到针对该寄生虫的抗体。与血清阳性患者相比，在该寄生虫抗体血清阴性的患者中，68.1%的患者IL-10水平显著升高。在南非的HIV患者中，该寄生虫的暴露水平较高，尽管阿米巴肝脓肿的患病率可能较低。这项研究表明，IL-10水平升高可能与阿米巴病风险降低有关。
