Ross Aisling M, Cahalane Rachel M, Walsh Darragh R, Grabrucker Andreas M, Marcar Lynnette, Mulvihill John J E
BioScience and BioEngineering Research (BioSciBer), Bernal Biomaterials, Bernal Institute, University of Limerick, V94 T9PX Limerick, Ireland.
School of Engineering, University of Limerick, V94 T9PX Limerick, Ireland.
Pharmaceutics. 2023 Jan 6;15(1):200. doi: 10.3390/pharmaceutics15010200.
Nanoparticles (NPs) represent an attractive strategy to overcome difficulties associated with the delivery of therapeutics. Knowing the optimal properties of NPs to address these issues could allow for improved in vivo responses. This work investigated NPs prepared from 5 materials of 3 sizes and 3 concentrations applied to a cell barrier model. The NPs permeability across a cell barrier and their effects on cell barrier integrity and cell viability were evaluated. The properties of these NPs, as determined in water (traditional) vs. media (realistic), were compared to cell responses. It was found that for all cellular activities, NP properties determined in media was the best predictor of the cell response. Notably, ZnO NPs caused significant alterations to cell viability across all 3 cell lines tested. Importantly, we report that the zeta potential of NPs correlates significantly with NP permeability and NP-induced changes in cell viability. NPs with physiological-based zeta potential of -12 mV result in good cell barrier penetration without considerable changes in cell viability.
纳米颗粒（NPs）是克服治疗药物递送相关困难的一种有吸引力的策略。了解纳米颗粒解决这些问题的最佳特性可以改善体内反应。这项工作研究了由5种材料制备的3种尺寸和3种浓度的纳米颗粒应用于细胞屏障模型的情况。评估了纳米颗粒跨细胞屏障的通透性及其对细胞屏障完整性和细胞活力的影响。将这些纳米颗粒在水（传统）与培养基（实际）中测定的特性与细胞反应进行了比较。结果发现，对于所有细胞活性，在培养基中测定的纳米颗粒特性是细胞反应的最佳预测指标。值得注意的是，氧化锌纳米颗粒在所有测试的3种细胞系中均引起细胞活力的显著改变。重要的是，我们报告纳米颗粒的zeta电位与纳米颗粒通透性和纳米颗粒诱导的细胞活力变化显著相关。具有-12 mV生理基础zeta电位的纳米颗粒可实现良好的细胞屏障穿透，而细胞活力无显著变化。
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