不同发育阶段玉米小粒突变体的表征及转录组分析

Characterization and Transcriptome Analysis of Maize Small-Kernel Mutant in Different Development Stages.

作者信息

Wang Jing, Wang Hongwu, Li Kun, Liu Xiaogang, Cao Xiaoxiong, Zhou Yuqiang, Huang Changling, Peng Yunling, Hu Xiaojiao

机构信息

College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.

National Engineering Research Center of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

出版信息

Plants (Basel). 2023 Jan 12;12(2):354. doi: 10.3390/plants12020354.

DOI:10.3390/plants12020354

PMID:36679067

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9866416/

Abstract

The kernel serves as a storage organ for various nutrients and determines the yield and quality of maize. Understanding the mechanisms regulating kernel development is important for maize production. In this study, a small-kernel mutant of maize was characterized. Cytological observation suggested that the development of the endosperm and embryo was arrested in in the early development stage. Biochemical tests revealed that the starch, zein protein, and indole-3-acetic acid (IAA) contents were significantly lower in compared with wild-type (WT). Consistent with the defective development phenotype, transcriptome analysis of the kernels 12 and 20 days after pollination (DAP) revealed that the starch, zein, and auxin biosynthesis-related genes were dramatically downregulated in . Genetic mapping indicated that the mutant was controlled by a recessive gene located on chromosome 2. Our results suggest that disrupted nutrition accumulation and auxin synthesis cause the defective endosperm and embryo development of .

摘要

玉米籽粒是多种营养物质的储存器官，决定着玉米的产量和品质。了解调控籽粒发育的机制对玉米生产至关重要。在本研究中，对一个玉米小籽粒突变体进行了表征。细胞学观察表明，胚乳和胚的发育在早期阶段就停止了。生化测试显示，与野生型（WT）相比，突变体中的淀粉、醇溶蛋白和吲哚 - 3 - 乙酸（IAA）含量显著降低。与发育缺陷表型一致，授粉后12天和20天（DAP）的籽粒转录组分析表明，突变体中淀粉、醇溶蛋白和生长素生物合成相关基因显著下调。遗传定位表明，该突变体由位于2号染色体上的一个隐性基因控制。我们的结果表明，营养积累和生长素合成的中断导致了突变体胚乳和胚的发育缺陷。