Potasman I, Araujo F G, Thulliez P, Desmonts G, Remington J S
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301.
J Clin Microbiol. 1987 Oct;25(10):1926-31. doi: 10.1128/jcm.25.10.1926-1931.1987.
The Sabin-Feldman dye test, the immunoglobulin M (IgM) immunosorbent agglutination assay, and the immunoblot technique were used to study the evolution of the antibody response to Toxoplasma gondii and to examine antigens of the organism recognized by antibodies in the sera of 12 congenitally infected infants and 7 mothers. In the sera of eight infants, a significant rise was noted in the dye test titers, while the serum of only one infant demonstrated a late increase in the IgM immunosorbent agglutination assay titer. In each infant and mother, antigens with approximate masses of 35,000 and 115,000 daltons were strongly recognized by IgG antibodies. An antigen(s) with an approximate mass of 4,000 daltons was recognized by IgM antibodies in the sera of each of the mothers but was recognized by antibodies in the sera of only two of the infants.
采用萨宾 - 费尔德曼染色试验、免疫球蛋白M（IgM）免疫吸附凝集试验和免疫印迹技术，研究对刚地弓形虫抗体反应的演变，并检测12名先天性感染婴儿和7名母亲血清中被抗体识别的该生物体抗原。在8名婴儿的血清中，染色试验滴度显著升高，而只有1名婴儿的血清在IgM免疫吸附凝集试验滴度中出现后期升高。在每个婴儿和母亲中，分子量约为35000和115000道尔顿的抗原被IgG抗体强烈识别。分子量约为4000道尔顿的一种或多种抗原在每位母亲的血清中被IgM抗体识别，但仅在2名婴儿的血清中被抗体识别。
