Desmonts G, Naot Y, Remington J S
J Clin Microbiol. 1981 Nov;14(5):486-91. doi: 10.1128/jcm.14.5.486-491.1981.
An immunoglobulin M (IgM)-immunosorbent agglutination assay (IgM-IS-AGA) was negative in all sera from individuals negative in the Sabin-Feldman dye test, in sera from individuals with chronic Toxoplasma infection, and in cord blood samples from uninfected infants. In contrast, all sera that were obtained from individuals with a recent history of acute Toxoplasma infection and from infants with congenital Toxoplasma infection and that were positive in both the dye test and the IgM-indirect fluorescent-antibody (IgM-IFA) test were positive in IgM-ISAGA. A total of 21 (67.7%) of 31 sera that were negative in the IgM-IFA test, despite being obtained from individuals with recently acquired Toxoplasma infection, and 8 (72.7%) of 11 sera that were negative in the IgM-IFA test and obtained from infants with congenital Toxoplasma infection were positive in IgM-ISAGA. The presence of rheumatoid factor, antinuclear antibodies, or both did not cause false-positive results in the IgM-ISAGA but did so in the IgM-IFA test. Thus, IgM-ISAGA in both more sensitive and more specific than the IgM-IFA test for detection of IgM antibodies to Toxoplasma gondii and, therefore, for the diagnosis of acute congenital and acquired Toxoplasma infections.
在所有Sabin-Feldman染料试验呈阴性的个体血清、慢性弓形虫感染个体的血清以及未感染婴儿的脐带血样本中，免疫球蛋白M（IgM）免疫吸附凝集试验（IgM-IS-AGA）均为阴性。相比之下，所有来自近期有急性弓形虫感染史个体以及先天性弓形虫感染婴儿且染料试验和IgM间接荧光抗体（IgM-IFA）试验均呈阳性的血清，在IgM-ISAGA中也呈阳性。尽管是从近期感染弓形虫的个体获得，但在IgM-IFA试验中呈阴性的31份血清中有21份（67.7%），以及从先天性弓形虫感染婴儿获得且在IgM-IFA试验中呈阴性的11份血清中有8份（72.7%）在IgM-ISAGA中呈阳性。类风湿因子、抗核抗体或两者同时存在不会导致IgM-ISAGA出现假阳性结果，但会导致IgM-IFA试验出现假阳性结果。因此，对于检测抗刚地弓形虫IgM抗体，进而诊断急性先天性和获得性弓形虫感染，IgM-ISAGA比IgM-IFA试验更敏感且更具特异性。
