Yang Yubin, Ding Jingyu, Zhu Xuanyan, Tian Zilu, Zhu Song
Department of Prosthetic Dentistry, Hospital of Stomatology, Jilin University, Changchun 130012, China.
Polymers (Basel). 2023 Jan 6;15(2):304. doi: 10.3390/polym15020304.
To solve the proble ms of composite restoration failure caused by secondary caries, this study reports a light curable antibacterial triclosan derivative (TCS-IH), which was synthesized and added to the existing commercial universal adhesive to achieve a long-term antibacterial effect The effect of mixing different mass percentages of TCS-IH on the bond strength of dentin was also investigated.TCS-IH was synthesized by solution polymerization and characterized by nuclear magnetic resonance hydrogen spectroscopy (1H NMR) and Fourier transform infrared (FTIR) spectroscopy. Two commercial universal adhesives, Single Bond Universal and All Bond Universal, were selected and used as the control group, and universal adhesives with different mass percentages (1 wt%, 3 wt%, 5 wt% and 7 wt%) of TCS-IH were used as the experimental group. The antibacterial properties were analysed by means of colony count experiments, biofilm formation detection, plotting of growth curves, biofilm metabolic activity detection, insoluble extracellular polysaccharide measurements and observations by confocal laser scanning microscopy and scanning electron microscopy (SEM). The effect of adhesives on biofilm formation, metabolism, extracellular matrix production, distribution of live and dead bacteria, and bacterial morphology of Streptococcus mutans (S. mutans) was analysed. The mechanical properties were evaluated by the degree of conversion and microtensile bonding strength under different conditions. Its biosafety was tested. We found that the addition of TCS-IH significantly improved the antibacterial performance of the universal adhesive, with the 5 wt% and 7 wt% groups showing the best antibacterial effect and effectively inhibiting the formation of biofilm. In addition, the adhesive strength test results showed that there was no statistical difference (p < 0.05) in the microtensile bond strength measured under various factors in all experimental groups except for the 7 wt% group in the self-etch bonding mode, and all of them had good biosafety. In summary, the 5 wt% group of antibacterial monomer TCS-IH was selected as the optimum addition to the universal adhesive to ensure the antimicrobial properties of the universal adhesive and the stability and durability of the adhesive interface. This study provides a reference for the clinical application of adhesives with antimicrobial activity to improve the stability and durability of adhesive restorations.
为解决继发龋导致复合树脂修复失败的问题，本研究报道了一种光固化抗菌三氯生衍生物（TCS-IH），其通过合成并添加到现有的商用通用粘结剂中以实现长期抗菌效果。还研究了混合不同质量百分比的TCS-IH对牙本质粘结强度的影响。TCS-IH通过溶液聚合合成，并通过核磁共振氢谱（1H NMR）和傅里叶变换红外（FTIR）光谱进行表征。选择两种商用通用粘结剂Single Bond Universal和All Bond Universal作为对照组，将含有不同质量百分比（1 wt%、3 wt%、5 wt%和7 wt%）TCS-IH的通用粘结剂作为实验组。通过菌落计数实验、生物膜形成检测、生长曲线绘制、生物膜代谢活性检测、不溶性胞外多糖测量以及共聚焦激光扫描显微镜和扫描电子显微镜（SEM）观察来分析抗菌性能。分析了粘结剂对变形链球菌（S. mutans）生物膜形成、代谢、细胞外基质产生、活菌和死菌分布以及细菌形态的影响。通过不同条件下的转化率和微拉伸粘结强度评估力学性能。测试了其生物安全性。我们发现添加TCS-IH显著提高了通用粘结剂的抗菌性能，5 wt%和7 wt%组显示出最佳抗菌效果并有效抑制了生物膜的形成。此外，粘结强度测试结果表明，除自酸蚀粘结模式下的7 wt%组外，所有实验组在各种因素下测量的微拉伸粘结强度均无统计学差异（p < 0.05），并且它们都具有良好的生物安全性。总之，选择5 wt%的抗菌单体TCS-IH作为通用粘结剂的最佳添加量，以确保通用粘结剂的抗菌性能以及粘结界面的稳定性和耐久性。本研究为具有抗菌活性的粘结剂在临床应用中提高粘结修复体的稳定性和耐久性提供了参考。
