Cho Kyou-Nam, Ouh In-Ohk, Park Young-Min, Park Min-Hee, Min Kyung-Min, Kang Hyang-Ju, Yun Su-Yeong, Song Jae-Young, Hyun Bang-Hun, Park Choi-Kyu, Choi Bo-Hwa, Lee Yoon-Hee
BioApplications Inc., Pohang-si 37668, Gyeongsangbuk-do, Republic of Korea.
Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea.
Vaccines (Basel). 2022 Dec 26;11(1):54. doi: 10.3390/vaccines11010054.
Porcine parvovirus (PPV) causes reproductive failure in sows, and vaccination remains the most effective means of preventing infection. The NADL-2 strain has been used as a vaccine for ~50 years; however, it does not protect animals against genetically heterologous PPV strains. Thus, new effective and safe vaccines are needed. In this study, we aimed to identify novel PPV1 strains, and to develop PPV1 subunit vaccines. We isolated and sequenced PPV1 genes from 926 pigs and identified ten PPV1 strains (belonging to Groups C, D and E). We selected the Group D PPV1-82 strain as a vaccine candidate because it was close to the highly pathogenic 27a strain. The PPV1-82 VP2 protein was produced in It formed virus-like particles and exhibited a 2 agglutination value. The PPV1-190313 strain (Group E), isolated from an aborted fetus, was used as the challenging strain because it was pathogenic. The unvaccinated sow miscarried at 8 days postchallenge, and mummified fetuses were all -positive. By contrast, pregnant sows vaccinated with PPV1-82 VP2 had 9-11 Log antibody titers and produced normal fetuses after PPV1-190313 challenge. These results suggest the PPV1-82 VP2 subunit vaccine protects pregnant sows against a genetically heterologous PPV1 strain by inducing neutralizing antibodies.
猪细小病毒(PPV)可导致母猪繁殖失败,接种疫苗仍然是预防感染的最有效手段。NADL - 2株作为疫苗已使用了约50年;然而,它不能保护动物免受基因异源的PPV毒株感染。因此,需要新的有效且安全的疫苗。在本研究中,我们旨在鉴定新型PPV1毒株,并开发PPV1亚单位疫苗。我们从926头猪中分离并测序了PPV1基因,鉴定出10株PPV1毒株(属于C、D和E组)。我们选择D组的PPV1 - 82毒株作为疫苗候选株,因为它与高致病性的27a毒株亲缘关系较近。PPV1 - 82 VP2蛋白在[具体表达系统未给出]中表达。它形成了病毒样颗粒,并表现出2的血凝效价。从流产胎儿中分离出的PPV1 - 190313毒株(E组)因其具有致病性,被用作攻毒毒株。未接种疫苗的母猪在攻毒后8天流产,木乃伊化胎儿均为阳性。相比之下,用PPV1 - 82 VP2疫苗接种的怀孕母猪在受到PPV1 - 190313攻毒后,抗体滴度为9 - 11 Log,并产下正常胎儿。这些结果表明,PPV1 - 82 VP2亚单位疫苗通过诱导中和抗体,可保护怀孕母猪免受基因异源的PPV1毒株感染。