State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Urumqi Customs Port Outpatient Department, Xinjiang International Travel Health Care Center, Urumqi 830011, China.
Viruses. 2022 Dec 23;15(1):49. doi: 10.3390/v15010049.
J paramyxovirus (JPV) is a rodent-borne Jeilongvirus isolated from moribund mice () with hemorrhagic lung lesions trapped in the 1972 in northern Queensland, Australia. The JPV antibodies have been detected in wild mice, wild rats, pigs, and human populations in Australia. Here, by next-generation sequencing (NGS), we detected JPV from in Shandong Province of China. Molecular detection of JPV was performed to survey to survey the infection among 66 species of wild small mammals collected from six eco-climate regions in China by applying JPV specific RT-PCR and sequencing. Altogether, 21 out of 3070 (0.68%) wild small mammals of four species were positive for JPV, including 5.26% (1/19) of , 3.76% (17/452) of , 1.67% (1/60) of , and 0.48% (2/421) of , which captured three eco-climate regions of China (northeastern China, northern China, and Inner Mongolia-Xinjiang). Sequence analysis revealed the currently identified JPV was clustered with other 14 Jeilongvirus members, and shared 80.2% and 89.2% identity with Australia's JPV partial RNA polymerase (L) and glycoprotein (G) genes, respectively. Phylogenetic analysis demonstrated the separation of three lineages of the current JPV sequences. Our results show three new hosts (, , and ) for JPV, most of which were widely distributed in China, and highlight the potential zoonotic transmission of JPV in humans. The detection of JPV in wild small mammals in China broaden the viral diversity, geographical distribution, and reservoir types of JPV. Future studies should prioritize determining the epidemiological characteristics of JPV, so that potential risks can be mitigated.
J 副黏病毒（JPV）是一种从北昆士兰州濒死的带有肺出血病变的老鼠（）中分离出来的啮齿动物携带的杰伊龙病毒。在澳大利亚，JPV 抗体已在野生老鼠、野生大鼠、猪和人群中检测到。在这里，通过下一代测序（NGS），我们从中国山东省检测到了 JPV。通过应用 JPV 特异性 RT-PCR 和测序，对从中国六个生态气候区采集的 66 种野生小型哺乳动物进行了 JPV 感染的分子检测。总共有 3070 只野生小型哺乳动物中的 21 只（0.68%）对 JPV 呈阳性，其中 4 种中有 5.26%（1/19）的（黑线姬鼠）、3.76%（17/452）的（黑线仓鼠）、1.67%（1/60）的（长尾仓鼠）和 0.48%（2/421）的（大仓鼠），这些动物来自中国的三个生态气候区（中国东北、华北和内蒙古-新疆）。序列分析表明，目前鉴定的 JPV 与其他 14 种杰伊龙病毒成员聚类在一起，与澳大利亚 JPV 部分 RNA 聚合酶（L）和糖蛋白（G）基因的同源性分别为 80.2%和 89.2%。系统发育分析表明，目前 JPV 序列分为三个谱系。我们的研究结果表明，有三种新的 JPV 宿主（黑线姬鼠、黑线仓鼠和长尾仓鼠），其中大多数在中国广泛分布，并强调了 JPV 在人类中的潜在人畜共患传播。在中国野生小型哺乳动物中检测到 JPV 拓宽了 JPV 的病毒多样性、地理分布和宿主类型。未来的研究应优先确定 JPV 的流行病学特征，以减轻潜在风险。
