Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei, PR China; Key Laboratory of Dermatology, Anhui Medical University, Hefei, PR China; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
Infect Genet Evol. 2020 Nov;85:104454. doi: 10.1016/j.meegid.2020.104454. Epub 2020 Jul 4.
Beilong virus (BeiV), a member of the newly recognized genus Jeilongvirus of family Paramyxoviridae, has been reported with limited geographic and host scopes, only in Hongkong, China and from two rat species. Here, by next-generation sequencing (NGS) on dominant wild small animal species in 4 provinces in China, we obtained a complete sequence of BeiV strain from Rattus norvegicus in Guangdong, neighboring HongKong, China. We then made an expanded epidemiological investigation in 11 provinces to obtain the geographic distribution and genetic features of this virus. Altogether 7168 samples from 2005 animals (1903 rodents, 100 shrews, 2 mustelidaes) that belonged to 33 species of Cricetidae, Muridae, Sciuridae and Dipodidae family of Rodentia, 3 species of Soricidae family of Soricomorpha, 2 species of Mustelidae family of Carnivora were examined by RT-PCR and sequencing. A positive rate of 3.7% (266/7168) was obtained that was detected from 22 animal species, including 5 species of Cricetidae family, 12 species of Muridae family, 2 species of Sciuridae family and 3 species of Soricidae family. Phylogenetic analyses based on 154 partial Large gene sequences grouped the current BeiV into two lineages, that were related to their geographic regions and animal hosts. Our study showed the wide distribution of BeiV in common species of wild rodents and shrews in China, highlighting the necessity of epidemiological study in wider regions.
贝隆病毒（BeiV）是副黏病毒科杰利昂病毒属的新成员，其地理和宿主范围有限，仅在中国香港地区的两种鼠种中被报道。在这里，通过对中国 4 个省份的主要野生小型动物物种进行下一代测序（NGS），我们从中国广东的褐家鼠中获得了贝隆病毒株的完整序列。随后，我们在中国 11 个省份进行了扩展的流行病学调查，以获得该病毒的地理分布和遗传特征。共对 2005 年的 7168 个样本（1903 只啮齿动物、100 只鼩鼱、2 只鼬科动物）进行了 RT-PCR 和测序，这些样本来自 33 种仓鼠科、鼠科、松鼠科和跳鼠科的啮齿动物，3 种鼩鼱科的食虫目，2 种鼬科的食肉目。从 22 种动物中检测到 3.7%（266/7168）的阳性率，包括 5 种仓鼠科、12 种鼠科、2 种松鼠科和 3 种鼩鼱科。基于 154 个部分大基因序列的系统发育分析将当前的贝隆病毒分为两个谱系，它们与地理区域和动物宿主有关。我们的研究表明，贝隆病毒在中国常见的野生啮齿动物和鼩鼱中广泛分布，突出了在更广泛地区进行流行病学研究的必要性。
