IRCCS San Raffaele Roma, Microbiology of Chronic Neuro-Degenerative Pathologies, 00163 Rome, Italy.
Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
Viruses. 2022 Dec 29;15(1):93. doi: 10.3390/v15010093.
In relation to the comment by Henriksen and Rinaldo, the authors intend to emphasize that before every experiment with SVGp12 cells they routinely test the cells for the absence of BKPyV contamination. The scientists can state that the SVGp12 cells used in their laboratory were not infected by BKPyV and that their results were also validated on the COS-7 cell line, which is permissive for JCPyV infection. Therefore, the overall findings of the study and its conclusions remain authentic. The authors recommend the necessity of carefully testing SVGp12 cells for BKPyV infection before use or, alternatively, in case of a first purchase; moreover, it is possible to choose different cell lines to avoid running into this unpleasant situation.
关于 Henriksen 和 Rinaldo 的评论，作者想强调的是，在使用 SVGp12 细胞进行每一项实验之前，他们都会例行检测细胞中是否存在 BKPyV 污染。研究人员可以声明，实验室使用的 SVGp12 细胞没有被 BKPyV 感染，他们的结果也在对 JCPyV 感染有易感性的 COS-7 细胞系上得到了验证。因此，研究的总体发现及其结论仍然是真实的。作者建议在使用 SVGp12 细胞之前，或者在首次购买时，仔细检测其是否感染 BKPyV；此外，还可以选择不同的细胞系，以避免出现这种不愉快的情况。
