Department of Research Center, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China.
Department of Research Center, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China; Department of Tumor Research Institute, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050017, People's Republic of China.
Biochem Biophys Res Commun. 2023 Feb 19;645:61-70. doi: 10.1016/j.bbrc.2023.01.043. Epub 2023 Jan 16.
Esophageal squamous cell carcinoma (ESCC) is a common malignancy with high morbidity and mortality. Although circular RNAs (circRNAs) play important roles in various cancers including ESCC, the role of the circRNA mannosidase alpha class 1A member 2 (circMAN1A2) in ESCC has been rarely studied. This study aimed to explore the role of circMAN1A2 in ESCC. CircMAN1A2 expression in ESCC tissues and cells was evaluated, and the relationship between circMAN1A2 expression and prognosis in patients with ESCC was analyzed. C-C chemokine ligand 5 (CCL5) was found to be a downstream target of circMAN1A2 by analysing the Agilent Microarray. Next, we performed in vitro and in vivo xenotransplantation assays to explore the role of circMAN1A2 in ESCC. We observed that high circMAN1A2 expression is associated with poor prognosis in patients with ESCC. Suppression of circMAN1A2 expression inhibits the proliferation, migration, and invasiveness of ESCC via regulating CCL5. Our results suggest that circMAN1A2 can promote the progression of ESCC by regulating CCL5. Thus, circMAN1A2 might be a novel diagnostic biomarker of ESCC, and targeting circMAN1A2 using inhibitors could be a potential therapeutic strategy to treat ESCC.
食管鳞状细胞癌 (ESCC) 是一种常见的恶性肿瘤，发病率和死亡率都很高。尽管环状 RNA（circRNA）在包括 ESCC 在内的各种癌症中发挥着重要作用，但 circRNA 甘露糖苷酶 alpha 类 1A 成员 2（circMAN1A2）在 ESCC 中的作用还很少被研究。本研究旨在探讨 circMAN1A2 在 ESCC 中的作用。评估了 circMAN1A2 在 ESCC 组织和细胞中的表达，并分析了 circMAN1A2 表达与 ESCC 患者预后的关系。通过分析 Agilent Microarray，发现 C-C 趋化因子配体 5（CCL5）是 circMAN1A2 的下游靶标。接下来，我们进行了体外和体内异种移植实验，以探讨 circMAN1A2 在 ESCC 中的作用。我们观察到，circMAN1A2 高表达与 ESCC 患者预后不良有关。通过调节 CCL5，抑制 circMAN1A2 的表达可抑制 ESCC 的增殖、迁移和侵袭。我们的结果表明，circMAN1A2 可通过调节 CCL5 促进 ESCC 的进展。因此，circMAN1A2 可能是 ESCC 的一种新型诊断生物标志物，使用抑制剂靶向 circMAN1A2 可能是治疗 ESCC 的一种潜在治疗策略。
