通过快速免疫电泳法检测一种新型肿瘤发生发展抗原的α2成分。

Measurement of the alpha 2 component of a novel oncodevelopmental antigen by a rapid electroimmunoassay.

作者信息

Nakai M, Atsumi M, Odagiri J, Arai M

机构信息

Department of Obstetrics and Gynaecology, Kitasato University School of Medicine, Kanagawa, Japan.

出版信息

J Immunol Methods. 1987 Nov 5;103(2):179-84. doi: 10.1016/0022-1759(87)90287-0.

DOI:10.1016/0022-1759(87)90287-0

PMID:3668256

Abstract

A method was required for the quantification of a novel oncodevelopmental antigen which increases in the serum of pregnant women as well as patients with ovarian cancer. The native antigen is composed of two components having alpha 2 and beta electrophoretic mobilities. An assay method, involving electroimmunodiffusion according to Laurell, was developed for the measurement of the alpha 2 component of the antigen. The method is sufficiently sensitive to determine significant differences in the levels of antigen in the serum of non-pregnant and pregnant subjects, and patients with ovarian cancer. In addition to routine quantitation, the technique will also be an indispensable analytical tool in the purification of the protein.

摘要

需要一种方法来定量一种新型的肿瘤发生发展抗原，这种抗原在孕妇以及卵巢癌患者的血清中含量会增加。天然抗原由具有α2和β电泳迁移率的两种成分组成。开发了一种根据劳雷尔方法进行免疫电泳扩散的检测方法，用于测量抗原的α2成分。该方法灵敏度足够高，能够确定非孕妇、孕妇以及卵巢癌患者血清中抗原水平的显著差异。除了常规定量外，该技术在蛋白质纯化过程中也将是一种不可或缺的分析工具。