Anthony F, Spencer K, Mason P, Masson G M, Price C P, Wood P J
Clin Chim Acta. 1980 Aug 4;105(2):287-95. doi: 10.1016/0009-8981(80)90471-4.
Four methods (radioimmunoassay, electroimmunoassay, laser nephelometry and kinetic immunoturbidimetry) have been evaluated for the measurement of proteins with pregnancy-specific beta1 glycoprotein immunoreactivity. The influence of two maternal plasma proteins with different electrophoretic mobilities (alpha2 and beta1) but with similar antigenic determinants has been assessed in each assay system. The radioimmunoassay method, which utilises a homogeneous preparation of pregnancy-specific beta1 glycoprotein for the preparation of iodinated tracer, and the kinetic immunoturbidimetric assay which measures antigen-antibody complex formation over the first minute of the reaction, were both considerably more specific for the beta1 component of pregnancy specific beta1 glycoprotein immunoreactivity than the laser nephelometric or electroimmunoassay methods.
已对四种方法（放射免疫测定法、电免疫测定法、激光散射比浊法和动态免疫比浊法）进行评估，以测定具有妊娠特异性β1糖蛋白免疫反应性的蛋白质。在每个检测系统中，均评估了两种具有不同电泳迁移率（α2和β1）但具有相似抗原决定簇的母体血浆蛋白的影响。放射免疫测定法利用妊娠特异性β1糖蛋白的均质制剂制备碘化示踪剂，动态免疫比浊法在反应的第一分钟测量抗原-抗体复合物的形成，这两种方法对妊娠特异性β1糖蛋白免疫反应性的β1成分均比激光散射比浊法或电免疫测定法更具特异性。
