采用3D打印技术制备的集成印刷脑源性神经营养因子刺激的人脐带间充质干细胞衍生外泌体/胶原蛋白/壳聚糖生物支架促进了创伤性脑损伤后神经网络的重塑。

Integrated printed BDNF-stimulated HUCMSCs-derived exosomes/collagen/chitosan biological scaffolds with 3D printing technology promoted the remodelling of neural networks after traumatic brain injury.

作者信息

Liu Xiaoyin, Zhang Jian, Cheng Xu, Liu Peng, Feng Qingbo, Wang Shan, Li Yuanyou, Gu Haoran, Zhong Lin, Chen Miao, Zhou Liangxue

机构信息

Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan 610041, China.

National Engineering Research Center for Biomaterials, College of Biomedical Engineering, Sichuan University, Chengdu, Sichuan 610064, China.

出版信息

Regen Biomater. 2022 Oct 26;10:rbac085. doi: 10.1093/rb/rbac085. eCollection 2023.

DOI:10.1093/rb/rbac085

PMID:36683754

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9847532/

Abstract

The restoration of nerve dysfunction after traumatic brain injury (TBI) faces huge challenges due to the limited self-regenerative abilities of nerve tissues. inductive recovery can be achieved utilizing biological scaffolds combined with endogenous human umbilical cord mesenchymal stem cells (HUCMSCs)-derived exosomes (MExos). In this study, brain-derived neurotrophic factor-stimulated HUCMSCs-derived exosomes (BMExos) were composited with collagen/chitosan by 3D printing technology. 3D-printed collagen/chitosan/BMExos (3D-CC-BMExos) scaffolds have excellent mechanical properties and biocompatibility. Subsequently, experiments showed that 3D-CC-BMExos therapy could improve the recovery of neuromotor function and cognitive function in a TBI model in rats. Consistent with the behavioural recovery, the results of histomorphological tests showed that 3D-CC-BMExos therapy could facilitate the remodelling of neural networks, such as improving the regeneration of nerve fibres, synaptic connections and myelin sheaths, in lesions after TBI.

摘要

由于神经组织的自我再生能力有限，创伤性脑损伤（TBI）后神经功能障碍的恢复面临巨大挑战。利用生物支架结合内源性人脐带间充质干细胞（HUCMSCs）衍生的外泌体（MExos）可实现诱导性恢复。在本研究中，通过3D打印技术将脑源性神经营养因子刺激的HUCMSCs衍生的外泌体（BMExos）与胶原蛋白/壳聚糖复合。3D打印的胶原蛋白/壳聚糖/BMExos（3D-CC-BMExos）支架具有优异的力学性能和生物相容性。随后，实验表明3D-CC-BMExos疗法可改善大鼠TBI模型中神经运动功能和认知功能的恢复。与行为恢复一致，组织形态学测试结果表明，3D-CC-BMExos疗法可促进TBI后病变部位神经网络的重塑，如改善神经纤维再生、突触连接和髓鞘形成。