Li Huizhu, Meng Xinxian, Sheng Huaixuan, Feng Sijia, Chen Yuzhou, Sheng Dandan, Sai Liman, Wang Yueming, Chen Mo, Wo Yan, Feng Shaoqing, Baharvand Hossein, Gao Yanglai, Li Yunxia, Chen Jun
Department of Sports Medicine, Sports Medicine Institute of Fudan University, Huashan Hospital, Fudan University, Shanghai 200040, China.
Department of Plastic and Reconstructive Surgery, School of Medicine, Shanghai Jiao Tong University, Shanghai Ninth People's Hospital, Shanghai 200011, China.
Regen Biomater. 2022 Dec 13;10:rbac102. doi: 10.1093/rb/rbac102. eCollection 2023.
The degradation of collagen in different body parts is a critical point for designing collagen-based biomedical products. Here, three kinds of collagens labeled by second near-infrared (NIR-II) quantum dots (QDs), including collagen with low crosslinking degree (LC), middle crosslinking degree (MC) and high crosslinking degree (HC), were injected into the subcutaneous tissue, muscle and joints of the mouse model, respectively, in order to investigate the degradation pattern of collagen by NIR-II live imaging. The results of NIR-II imaging indicated that all tested collagens could be fully degraded after 35 days in the subcutaneous tissue, muscle and joints of the mouse model. However, the average degradation rate of subcutaneous tissue ( = 0.13) and muscle ( = 0.23) was slower than that of the joints (shoulder: = 0.42, knee: = 0.55). Specifically, the degradation rate of HC ( = 0.13) was slower than LC ( = 0.30) in muscle, while HC showed the fastest degradation rate in the shoulder and knee joints. In summary, NIR-II imaging could precisely identify the degradation rate of collagen. Moreover, the degradation rate of collagen was more closely related to the implanted body parts rather than the crosslinking degree of collagen, which was slower in the subcutaneous tissue and muscle compared to the joints in the mouse model.
不同身体部位胶原蛋白的降解是设计基于胶原蛋白的生物医学产品的关键要点。在此，将三种由近红外二区（NIR-II）量子点（QDs）标记的胶原蛋白，包括低交联度（LC）、中等交联度（MC）和高交联度（HC）的胶原蛋白，分别注射到小鼠模型的皮下组织、肌肉和关节中，以通过NIR-II活体成像研究胶原蛋白的降解模式。NIR-II成像结果表明，在小鼠模型的皮下组织、肌肉和关节中，所有测试的胶原蛋白在35天后均可完全降解。然而，皮下组织（=0.13）和肌肉（=0.23）的平均降解速率比关节（肩部：=0.42，膝部：=0.55）慢。具体而言，HC在肌肉中的降解速率（=0.13）比LC（=0.30）慢，而HC在肩部和膝关节中显示出最快的降解速率。总之，NIR-II成像能够精确识别胶原蛋白的降解速率。此外，胶原蛋白的降解速率与植入的身体部位而非胶原蛋白的交联度关系更为密切，在小鼠模型中，皮下组织和肌肉中的降解速率比关节慢。
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