Dong Shixian, Feng Sijia, Chen Yuzhou, Chen Mo, Yang Yimeng, Zhang Jian, Li Huizhu, Li Xiaotong, Ji Liang, Yang Xing, Hao Yuefeng, Chen Jun, Wo Yan
Department of Anatomy and Physiology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Department of Sports Medicine, Sports Medicine Institute of Fudan University, Huashan Hospital, Fudan University, Shanghai, China.
Front Chem. 2021 Jul 14;9:676928. doi: 10.3389/fchem.2021.676928. eCollection 2021.
Peripheral nerve injury gives rise to devastating conditions including neural dysfunction, unbearable pain and even paralysis. The therapeutic effect of current treatment for peripheral nerve injury is unsatisfactory, resulting in slow nerve regeneration and incomplete recovery of neural function. In this study, nerve suture combined with ADSCs injection was adopted in rat model of sciatic nerve injury. Under real-time visualization of the injected cells with the guidance of NIR-II fluorescence imaging , a spatio-temporal map displaying cell migration from the proximal injection site (0 day post-injection) of the nerve to the sutured site (7 days post-injection), and then to the distal section (14 days post-injection) was demonstrated. Furthermore, the results of electromyography and mechanical pain threshold indicated nerve regeneration and functional recovery after the combined therapy. Therefore, in the current study, the observed ADSCs migration , electrophysiological examination results and pathological changes all provided robust evidence for the efficacy of the applied treatment. Our approach of nerve suture combined with ADSCs injection in treating peripheral nerve injury under real-time NIR-II imaging monitoring added novel insights into the treatment for peripheral nerve injury, thus further enhancing in-depth understanding of peripheral nerve regeneration and the mechanism behind.
周围神经损伤会引发包括神经功能障碍、难以忍受的疼痛甚至瘫痪等严重后果。目前针对周围神经损伤的治疗效果并不理想，导致神经再生缓慢且神经功能恢复不完全。在本研究中，在大鼠坐骨神经损伤模型中采用了神经缝合联合脂肪干细胞（ADSCs）注射的方法。在近红外二区（NIR-II）荧光成像的引导下对注射的细胞进行实时可视化，展示了从神经近端注射部位（注射后0天）到缝合部位（注射后7天），再到远端（注射后14天）的细胞迁移时空图。此外，肌电图和机械性疼痛阈值的结果表明联合治疗后神经再生和功能恢复。因此，在本研究中，观察到的脂肪干细胞迁移、电生理检查结果和病理变化均为所应用治疗方法的疗效提供了有力证据。我们在实时近红外二区成像监测下将神经缝合与脂肪干细胞注射相结合治疗周围神经损伤的方法，为周围神经损伤的治疗增添了新的见解，从而进一步加深了对周围神经再生及其背后机制的深入理解。
