Sun Bo, Meng Xianhui, Li Yumin, Li Yanlong, Liu Rui, Xiao Zhongdang
State key laboratory of bioelectronics, school of biological science and medical engineering, Southeast University, Nanjing, China.
Shandong Electric Power Central Hospital, Jinan, China.
Front Cell Dev Biol. 2023 Jan 4;10:1042609. doi: 10.3389/fcell.2022.1042609. eCollection 2022.
Aging is accompanied with progressive deterioration of immune responses and tissue's function. Using 12-month-old mice as model, we showed that conditioned medium of human cord blood mesenchymal stem cells (CBMSC-CM) significantly reduced the population percentage of CD3CD335 NK and CD4CD25 regulatory T-cells in peripheral blood. The CBMSC-CM administration also increased naïve T-cells number and restored the ratio of naïve to memory T-cells in CD4 T-cells population. These results indicated that CBMSC-CM improved the immune response efficiency of aged mice. Moreover, we also found CBMSC-CM treatment significantly reduced the number of senescenT-cells in kidney tissues. Finally, we demonstrated that CBMSC-CM remarkably attenuated hydrogen peroxide triggered T-cell response and ameliorated oxidative stress induced cellular senescence. All of these data suggest a prominent anti-aging effect of secretome of CBMSCs.
衰老伴随着免疫反应和组织功能的渐进性衰退。以12月龄小鼠为模型，我们发现人脐带血间充质干细胞条件培养基（CBMSC-CM）显著降低了外周血中CD3CD335自然杀伤细胞和CD4CD25调节性T细胞的群体百分比。给予CBMSC-CM还增加了初始T细胞数量，并恢复了CD4 T细胞群体中初始T细胞与记忆T细胞的比例。这些结果表明CBMSC-CM提高了老年小鼠的免疫反应效率。此外，我们还发现CBMSC-CM处理显著减少了肾组织中衰老T细胞的数量。最后，我们证明CBMSC-CM显著减弱了过氧化氢触发的T细胞反应，并改善了氧化应激诱导的细胞衰老。所有这些数据表明CBMSCs分泌组具有显著的抗衰老作用。
