Effect of (L) Extract on Adipogenesis, Lipolysis and Cholesterol Esterase in 3T3-L1 Adipocyte Cell Lines.

(L) is an Indian traditional plant used for the treatment of diabetes and heart diseases. The present study is to evaluate the functional of leaf extract as insulin like character to inhibit lipolysis and stimulates Adipogenesis activity. The ability of the leaf extract in anti-obesity effect of Adipogenesis, lipolysis and cholesterol esterase functions can be predicted by using 3T3-L1 adipocyte cell lines. Substances were isolated from leaves and the double filtered crude sample were used for Adipogenesis, lipolysis and cholesterol esterase activity using 3T3-L1 adipocytes at different concentrations. We used differential media-I, differential media-II and maintenance media (MM1) at concentrations of 20, 40, 60, 80, 100, 200 and 400 µg/mL respectively. In addition to the extract, there is a significance increase in glycerol release ( < 0.001) compared with crude and reference compounds. Cholesterol esterase activity predicts the IC = 27.11 µg/mL of orlistat positive control compare with IC = 8.158 µg/mL of crude extract. Based on the observation, leaf extract can promotes lipolysis and differentiated adipocytes. It is potentially used as adjuvant in the treatment of Type 2 diabetes.