Lakshminarayana Lavanya, Veeraraghavan V, Gouthami Kuruvalli, Srihari Renuka, Chowdadenahalli Nagaraja Prashantha
Department of Biochemistry, School of Applied Sciences, REVA University, Bangalore, 560064 India.
Department of Biochemistry, Maharani Lakshmi Ammanni College for Women, Bangalore, 560012 India.
Indian J Clin Biochem. 2023 Jan;38(1):22-32. doi: 10.1007/s12291-022-01022-2. Epub 2022 Apr 6.
(L) is an Indian traditional plant used for the treatment of diabetes and heart diseases. The present study is to evaluate the functional of leaf extract as insulin like character to inhibit lipolysis and stimulates Adipogenesis activity. The ability of the leaf extract in anti-obesity effect of Adipogenesis, lipolysis and cholesterol esterase functions can be predicted by using 3T3-L1 adipocyte cell lines. Substances were isolated from leaves and the double filtered crude sample were used for Adipogenesis, lipolysis and cholesterol esterase activity using 3T3-L1 adipocytes at different concentrations. We used differential media-I, differential media-II and maintenance media (MM1) at concentrations of 20, 40, 60, 80, 100, 200 and 400 µg/mL respectively. In addition to the extract, there is a significance increase in glycerol release ( < 0.001) compared with crude and reference compounds. Cholesterol esterase activity predicts the IC = 27.11 µg/mL of orlistat positive control compare with IC = 8.158 µg/mL of crude extract. Based on the observation, leaf extract can promotes lipolysis and differentiated adipocytes. It is potentially used as adjuvant in the treatment of Type 2 diabetes.
(L)是一种用于治疗糖尿病和心脏病的印度传统植物。本研究旨在评估叶提取物类似胰岛素的特性，以抑制脂肪分解并刺激脂肪生成活性。叶提取物在脂肪生成、脂肪分解和胆固醇酯酶功能的抗肥胖作用方面的能力可以通过使用3T3-L1脂肪细胞系来预测。从叶中分离出物质，将双重过滤后的粗样品用于不同浓度下对3T3-L1脂肪细胞的脂肪生成、脂肪分解和胆固醇酯酶活性研究。我们分别使用浓度为20、40、60、80、100、200和400μg/mL的分化培养基-I、分化培养基-II和维持培养基(MM1)。除提取物外，与粗品和参考化合物相比，甘油释放量显著增加(<0.001)。胆固醇酯酶活性预测奥利司他阳性对照的IC=27.11μg/mL，而粗提取物的IC=8.158μg/mL。基于这些观察结果，叶提取物可以促进脂肪分解和分化的脂肪细胞。它有可能用作2型糖尿病治疗的辅助药物。
