Tseng Sung-Hui, Lee Chia-Jung, Chen Shih-Han, Chen Chao-Hsin, Tsai Po-Wei, Hsieh Ming-Shium, Chu Jan-Show, Wang Ching-Chiung
Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei, Taiwan.
Department of Physical Medicine and Rehabilitation, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
J Tradit Complement Med. 2022 Oct 14;13(1):51-61. doi: 10.1016/j.jtcme.2022.10.003. eCollection 2023 Jan.
Du-Huo-Ji-Sheng-Tang (DHJST) is a Chinese herbal formula used for arthralgia and arthritis treatment clinically. This study aims to evaluate the joint-protecting efficacy of DHJST and to identify the active constituents as the evaluation marker.
DHJST can be categorized into three recipes: Blood-tonifying-herbs Si-Wu-Tang (SWT), Wind-dampness-dispelling-herbs (WDH) and Qi-tonifying-herbs (TH). All formulas were used to explore the joint-protecting efficacies.
: Firstly, DHJST could decrease the arthritis progression in the monosodium-iodoacetate-induced rat and cure arthritis in the type II collagenase-induced rat. Further, in lipopolysaccharide-stimulated RAW 264.7 cells, DHJST, TH and (CC), an ingredient in TH, were the most potent nitric oxide (NO) and prostaglandin E (PGE) inhibitors. The major components, cinnamic aldehyde, showed the strongest NO and PGE inhibition. Up-regulated inducible NO synthase (iNOS) and cyclooxygenase-2 were inhibited by DHJST, TH, CC, and cinnamic aldehyde. In interleukin-1β-stimulated primary chondrocytes, upregulated iNOS was inhibited by DHJST, TH, , and cinnamic aldehyde. Upregulated matrix metalloprotease-13 was only inhibited by DHJST and TH and (EU) extract. Results suggest that DHJST presented joint-protective and cure arthritis effects. TH presented equal joint-protective effects as DHJST. The major anti-inflammatory ingredient in TH was in TH. And cinnamic aldehyde was the potent anti-inflammatory active compound in Therefore, this study may facilitate the modern use of DHJST with TH as a simplified version but equally effective anti-osteoarthritic agents with cinnamic aldehyde as a quality control marker of DHJST and TH in osteoarthritis prevention or treatment.
独活寄生汤（DHJST）是一种临床上用于治疗关节痛和关节炎的中药方剂。本研究旨在评估独活寄生汤的关节保护功效，并确定其活性成分作为评价指标。
独活寄生汤可分为三个配方：补血药四物汤（SWT）、祛风湿药（WDH）和补气药（TH）。所有配方均用于探究关节保护功效。
首先，独活寄生汤可减缓碘乙酸钠诱导的大鼠关节炎进展，并治愈II型胶原酶诱导的大鼠关节炎。此外，在脂多糖刺激的RAW 264.7细胞中，独活寄生汤、TH以及TH中的一种成分（CC）是最有效的一氧化氮（NO）和前列腺素E（PGE）抑制剂。主要成分肉桂醛表现出最强的NO和PGE抑制作用。独活寄生汤、TH、CC和肉桂醛可抑制诱导型一氧化氮合酶（iNOS）和环氧化酶-2的上调。在白细胞介素-1β刺激的原代软骨细胞中，独活寄生汤、TH、[成分名称未给出]和肉桂醛可抑制iNOS的上调。上调的基质金属蛋白酶-13仅被独活寄生汤、TH和[提取物名称未给出]（EU）提取物抑制。结果表明，独活寄生汤具有关节保护和治愈关节炎的作用。TH表现出与独活寄生汤同等的关节保护作用。TH中的主要抗炎成分是TH中的[成分名称未给出]。肉桂醛是[具体部位未明确]中有效的抗炎活性化合物。因此，本研究可能有助于独活寄生汤与TH作为简化版但同样有效的抗骨关节炎药物的现代应用，以肉桂醛作为独活寄生汤和TH在骨关节炎预防或治疗中的质量控制指标。
