Kumaraswamy Monika, Coady Alison, Szubin Richard, Martin Thomas Cs, Palsson Bernhard, Nizet Victor, Monk Jonathan M
Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), University of California San Diego, La Jolla, CA 92093, USA.
Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA.
Curr Res Microb Sci. 2023 Jan 9;4:100180. doi: 10.1016/j.crmicr.2023.100180. eCollection 2023.
Comprehensive whole genome sequencing (WGS) with hybrid assembly of a multi-drug resistant (MDR) (CA) isolate causing cerebral abscess was performed using Illumina paired end and Oxford Nanopore long read technologies. The innovative technologies utilized here enabled us to resolve fragmented assemblies, and implement comprehensive and detailed genomic analyses involved in antifungal resistance of Functionally important genes (MDR1, CDR2 and SQN2) involved in antifungal resistance were identified and a phylogenetic analysis of the clinical isolate was performed. Additionally, our clinical isolate was found to share 4 single nucleotide polymorphisms with two other sequenced strains of MDR (381 and 386) including translation elongation factor EF1α and EF3, ATPase activity associated proteins, and the lysine tRNA ligase.
使用Illumina双端测序和牛津纳米孔长读长技术，对一株引起脑脓肿的多重耐药（MDR） （CA）分离株进行了全基因组测序（WGS）及混合组装。此处使用的创新技术使我们能够解决片段化组装问题，并对涉及抗真菌耐药性的功能重要基因进行全面而详细的基因组分析。鉴定出了与抗真菌耐药性相关的功能重要基因（MDR1、CDR2和SQN2），并对该临床分离株进行了系统发育分析。此外，我们发现该临床分离株与另外两株已测序的MDR菌株（381和386）共有4个单核苷酸多态性，包括翻译延伸因子EF1α和EF3、与ATP酶活性相关的蛋白质以及赖氨酸tRNA连接酶。
