Zhang Ya, Guo Jing, Gao Yueying, Li Si, Pan Tao, Xu Gang, Li Xia, Li Yongsheng, Yang Jun
Key Laboratory of Tropical Translational Medicine of Ministry of Education, College of Biomedical Information and Engineering, Hainan Women and Children's Medical Center, Hainan Medical University, Haikou 571199, China.
College of Bioinformatics Science and Technology, Harbin Medical University, Harbin 150081, China.
Heliyon. 2023 Jan 4;9(1):e12690. doi: 10.1016/j.heliyon.2022.e12690. eCollection 2023 Jan.
Dengue infection is one of the most prevalent arthropod-borne viral diseases, which can result in severe complications. Identification of genes and long non-coding RNAs (lncRNAs) involved in dengue infection would help in deciphering potential mechanisms responsible for the disease progression. We comprehensively analyzed the dynamic transcriptome during dengue disease progression and identified critical genes and lncRNAs with expression perturbations. Our findings revealed that the expression of genes (i.e., CCR10 and GNG7) and lncRNAs (i.e., CTBP1-AS and MAFG-AS1) were potentially regulated by m6A RNA methylation. Interestingly, dengue viral proteins prevalently interact with genes or lncRNAs with expression perturbations, which are involved in cell cycle, inflammation signaling pathways and immune response. Dynamically expressed genes and lncRNAs were likely to locate in the central regions of human protein-protein network, which play crucial roles in mediating signaling spread and helping viral replication. Immune microenvironments analysis revealed that plasma cells levels were increased and T cells infiltrations were decreased during dengue disease progression. Dynamically expressed genes and lncRNAs were correlated with immune cell infiltrations. Moreover, network analysis reveals the associations between dengue viral infections and human complex diseases (i.e., digestive diseases and neoplasms). Our comprehensive transcriptome analysis of dengue disease progression identified potential gene and lncRNA biomarkers, providing novel insights for understanding the pathogenesis of and developing effective therapeutic strategies for dengue infection.
登革热感染是最常见的节肢动物传播的病毒性疾病之一，可导致严重并发症。鉴定参与登革热感染的基因和长链非编码RNA（lncRNA）将有助于阐明导致疾病进展的潜在机制。我们全面分析了登革热疾病进展过程中的动态转录组，并鉴定了表达受到干扰的关键基因和lncRNA。我们的研究结果表明，基因（即CCR10和GNG7）和lncRNA（即CTBP1-AS和MAFG-AS1）的表达可能受m6A RNA甲基化调控。有趣的是，登革热病毒蛋白普遍与表达受到干扰的基因或lncRNA相互作用，这些基因和lncRNA参与细胞周期、炎症信号通路和免疫反应。动态表达的基因和lncRNA可能位于人类蛋白质-蛋白质网络的中心区域，在介导信号传播和帮助病毒复制中起关键作用。免疫微环境分析显示，在登革热疾病进展过程中浆细胞水平升高，T细胞浸润减少。动态表达的基因和lncRNA与免疫细胞浸润相关。此外，网络分析揭示了登革热病毒感染与人类复杂疾病（即消化系统疾病和肿瘤）之间的关联。我们对登革热疾病进展的全面转录组分析鉴定了潜在的基因和lncRNA生物标志物，为理解登革热感染的发病机制和制定有效的治疗策略提供了新的见解。
