使用耐氧氧化还原酶的氢燃料一氧化碳还原反应。
Hydrogen-fueled CO reduction using oxygen-tolerant oxidoreductases.
作者信息
Cha Jaehyun, Bak Hyeonseon, Kwon Inchan
机构信息
School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, South Korea.
Research Center for Innovative Energy and Carbon Optimized Synthesis for Chemicals (Inn-ECOSysChem), Gwangju Institute of Science and Technology (GIST), Gwangju, South Korea.
出版信息
Front Bioeng Biotechnol. 2023 Jan 5;10:1078164. doi: 10.3389/fbioe.2022.1078164. eCollection 2022.
Hydrogen gas obtained from cheap or sustainable sources has been investigated as an alternative to fossil fuels. By using hydrogenase (Hase) and formate dehydrogenase (FDH), H and CO gases can be converted to formate, which can be conveniently stored and transported. However, developing an enzymatic process that converts H and CO obtained from cheap sources into formate is challenging because even a very small amount of O included in the cheap sources damages most Hases and FDHs. In order to overcome this limitation, we investigated a pair of oxygen-tolerant Hase and FDH. We achieved the cascade reaction between Hase from H16 (ReSH) and FDH from (RcFDH) to convert H and CO to formate using regeneration of NAD/NADH in the presence of O.
从廉价或可持续来源获得的氢气已被作为化石燃料的替代品进行研究。通过使用氢化酶（Hase）和甲酸脱氢酶（FDH），氢气和一氧化碳气体可以转化为甲酸，甲酸便于储存和运输。然而，开发一种将从廉价来源获得的氢气和一氧化碳转化为甲酸的酶促过程具有挑战性，因为即使廉价来源中包含的极少量氧气也会损害大多数氢化酶和甲酸脱氢酶。为了克服这一限制，我们研究了一对耐氧氢化酶和甲酸脱氢酶。我们实现了来自嗜热栖热放线菌（ReSH）的氢化酶和来自嗜热栖热放线菌（RcFDH）的甲酸脱氢酶之间的级联反应，以在有氧存在的情况下利用NAD/NADH的再生将氢气和一氧化碳转化为甲酸。
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