Vasudevan Anjana Cherath, Sridhar Nekkanti, Bhat Vinutha, Bhat Meghana
Department of Pediatric and Preventive Dentistry, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Department of Biochemistry, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Contemp Clin Dent. 2022 Oct-Dec;13(4):315-321. doi: 10.4103/ccd.ccd_276_21. Epub 2022 Nov 3.
Factors in the biofilm influence the induction and advancement of the carious process. This study was done to relate and assess the levels of enzyme carbonic anhydrase VI, total protein, and antioxidants (superoxide dismutase [SOD], catalase, lipid peroxidase, and uric acid) in caries-free and caries-active children.
This case-control study comprised 60 children of age group 6-12 years who were evaluated for decayed missing filled teeth (dmft)/DMFT criteria and distributed into two groups: Group 1 - caries active (case) and Group 2 - caries free (control) for saliva collection with 30 participants in each of the above groups. Stimulated saliva was obtained, and the samples were then evaluated using biochemical lab tests. The data were then statistically evaluated using independent -test.
Catalase in the caries-free group was significantly higher, but the concentration of carbonic anhydrase (CAVI) enzyme, total protein, and other antioxidant enzyme activity was enhanced in caries-active children in which uric acid demonstrated a statistically significant difference with higher levels in caries-active group.
There is an increased concentration of CAVI enzyme in caries-active group and total protein showed a linear relation with caries activity. Antioxidant parameters such as SOD and lipid peroxidase were increased with caries activity. Uric acid was significantly higher in the caries-active group, whereas catalase showed an indirect relation with dental caries. Significant variations in the levels of these parameters imply that the levels of these components of saliva can act as strong markers of caries status in children.
生物膜中的因素会影响龋病进程的诱发和发展。本研究旨在关联并评估无龋和患龋儿童体内碳酸酐酶VI、总蛋白及抗氧化剂（超氧化物歧化酶[SOD]、过氧化氢酶、脂质过氧化物酶和尿酸）的水平。
本病例对照研究纳入了60名6 - 12岁的儿童，根据龋失补牙数（dmft）/恒牙龋失补牙数（DMFT）标准进行评估，并分为两组：第1组——患龋组（病例组）和第2组——无龋组（对照组），每组30名参与者，用于收集唾液。收集刺激唾液后，使用生化实验室检测对样本进行评估。然后使用独立样本t检验对数据进行统计学评估。
无龋组的过氧化氢酶显著更高，但患龋儿童体内碳酸酐酶（CAVI）、总蛋白及其他抗氧化酶活性增强，其中尿酸在患龋组中水平更高，差异具有统计学意义。
患龋组中CAVI酶浓度升高，总蛋白与龋病活动呈线性关系。超氧化物歧化酶和脂质过氧化物酶等抗氧化参数随龋病活动增加。患龋组中尿酸显著更高，而过氧化氢酶与龋齿呈间接关系。这些参数水平的显著差异表明，唾液中这些成分的水平可作为儿童龋病状况的有力指标。
