Piracicaba Dental School, Pediatric Dentistry Department, University of Campinas, 901 Limeira Av, 13414-903 Piracicaba, São Paulo, Brazil.
Laboratory of Microbiology and Molecular Biology, University São Francisco, 218 São Francisco de Assis Av, 12916-900 Bragança Paulista, São Paulo, Brazil.
Int J Mol Sci. 2019 May 30;20(11):2673. doi: 10.3390/ijms20112673.
This study investigated pH, activity and concentration of carbonic anhydrase VI (CA VI) in dental biofilm of caries and caries-free children of 7-9 years old. Seventy-four children were selected and divided into two groups. The caries diagnosis was performed according to the WHO criteria, including the early caries lesion. After biofilm collection and pH determination, CA VI concentration and activity were determined by ELISA and Zimography respectively. The data were submitted to a Mann-Whitney test and to Pearson and Spearman correlation analyses. Means and standard deviations of dental caries for the caries group were of 3.162 ± 1.385. The biofilm pH was significantly higher in the caries-free group. The CA VI activity was significantly higher in biofilm of children with caries. The CA VI concentration was significantly higher in biofilm of caries-free children. In caries-free children, there was a moderate negative correlation between CA VI activity and concentration in dental biofilm as well as between pH and CA VI activity. A negative correlation between biofilm pH and CA VI concentration was found in the caries group. In conclusion, CA VI was shown to be more active in the biofilm of school children with caries in order to contribute to neutralization of biofilm acid.
本研究调查了龋病和无龋儿童 7-9 岁的牙菌斑的 pH 值、碳酸酐酶 VI（CA VI）活性和浓度。选择了 74 名儿童并将其分为两组。根据世界卫生组织的标准进行龋齿诊断，包括早期龋齿病变。在收集生物膜并测定 pH 值后，通过 ELISA 和 Zimography 分别测定 CA VI 浓度和活性。数据提交给曼-惠特尼检验和皮尔逊和斯皮尔曼相关性分析。龋病组的平均龋齿和标准差分别为 3.162±1.385。无龋组的生物膜 pH 值显著更高。龋齿儿童的 CA VI 活性显著更高。无龋儿童的 CA VI 浓度显著更高。在无龋儿童中，牙菌斑中 CA VI 活性和浓度之间以及 pH 值和 CA VI 活性之间存在中度负相关。在龋病组中发现生物膜 pH 值与 CA VI 浓度之间存在负相关。总之，CA VI 在患有龋齿的学龄儿童的生物膜中表现出更高的活性，以有助于中和生物膜酸。
