Yefet Ron, Friedel Nadav, Tamir Hadas, Polonsky Ksenia, Mor Michael, Cherry-Mimran Lilach, Taleb Eyal, Hagin David, Sprecher Eli, Israely Tomer, Freund Natalia T
Department of Microbiology and Clinical Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Division of Dermatology, Tel-Aviv Sourasky Medical Center, Tel Aviv, Israel.
iScience. 2023 Feb 17;26(2):105957. doi: 10.1016/j.isci.2023.105957. Epub 2023 Jan 13.
Monkeypox virus (MPXV) resides in two forms; mature and enveloped, and depending on it, distinct proteins are displayed on the viral surface. Here, we expressed two MPXV antigens from the mature, and one from the enveloped form, and tested their reactivity to sera of 11 MPXV recoverees while comparing to sera from recently and past vaccinated individuals. 8 out of 11 recoverees exhibited detectable neutralization levels against Vaccinia Lister. Sera from all recoverees bound strongly to A35R and H3L antigens. Moreover, the responses to A35R were significantly higher within the recoverees compared to both recently and past vaccinated donors. Lastly, A35R- and H3L-specific IgG B cells ranging from 0.03-0.46% and 0.11-0.36%, respectively, were detected in all recoverees (A35R), and in 9 out of 11 recoverees (H3L). Therefore, A35R and H3L represent MPXV immune targets and could be used in a heat-inactivated serological ELISA for the identification of recent MPXV infection.
猴痘病毒（MPXV）以两种形式存在：成熟形式和包膜形式，据此，不同的蛋白质会呈现在病毒表面。在此，我们表达了来自成熟形式的两种MPXV抗原以及来自包膜形式的一种抗原，并检测了它们与11名MPXV康复者血清的反应性，同时与近期和既往接种疫苗个体的血清进行比较。11名康复者中有8人对痘苗病毒李斯特株表现出可检测到的中和水平。所有康复者的血清均与A35R和H3L抗原强烈结合。此外，与近期和既往接种疫苗的供者相比，康复者体内对A35R的反应显著更高。最后，在所有康复者（A35R）以及11名康复者中的9人（H3L）中分别检测到了比例范围为0.03 - 0.46%和0.11 - 0.36%的A35R特异性和H3L特异性IgG B细胞。因此，A35R和H3L代表MPXV免疫靶点，可用于热灭活血清学ELISA以鉴定近期的MPXV感染。
