Zhang Heao, Liu Qingyang, Liang Qing, Wang Boxiang, Chen Zixi, Wang Jiangxin
Whittle School and Studios, Shenzhen, Guangdong, China.
Shenzhen Link Spider Technology Co., Ltd., Shenzhen, China.
Front Microbiol. 2023 Jan 4;13:1091502. doi: 10.3389/fmicb.2022.1091502. eCollection 2022.
Tardigrades, known colloquially as water bears or moss piglets, are diminutive animals capable of surviving many extreme environments, even been exposed to space in low Earth orbit. Recently termed tardigrade disordered proteins (TDPs) include three families as cytoplasmic-(CAHS), secreted-(SAHS), and mitochondrial-abundant heat soluble (MAHS) proteins. How these tiny animals survive these stresses has remained relatively mysterious. Cyanobacteria cast attention as a "microbial factory" to produce biofuels and high-value-added chemicals due to their ability to photosynthesis and CO sequestration. We explored a lot about biofuel stress and related mechanisms in PCC 6803. The previous studies show that CAHS protein heterogenous expression in bacteria, yeast, and human cells increases desiccation tolerance in these hosts. In this study, the expression of three CAHS proteins in cyanobacterium was found to affect the tolerance to biofuels, while the tolerance to Cd and Zn were slightly affected in several mutants. A quantitative transcriptomics approach was applied to decipher response mechanisms at the transcriptional level further.
缓步动物，俗称水熊或苔藓小猪，是一种微小的动物，能够在许多极端环境中生存，甚至能在近地轨道暴露于太空环境。最近被称为缓步动物无序蛋白（TDPs）的包括三个家族，即细胞质丰富热溶性蛋白（CAHS）、分泌性丰富热溶性蛋白（SAHS）和线粒体丰富热溶性蛋白（MAHS）。这些微小的动物如何在这些压力下生存仍然相对神秘。蓝细菌因其光合作用和固碳能力而作为生产生物燃料和高附加值化学品的“微生物工厂”受到关注。我们对集胞藻PCC 6803中的生物燃料胁迫及相关机制进行了大量探索。先前的研究表明，CAHS蛋白在细菌、酵母和人类细胞中的异源表达提高了这些宿主的耐干燥能力。在本研究中，发现三种CAHS蛋白在蓝细菌中的表达影响了对生物燃料的耐受性，而几个突变体对镉和锌的耐受性受到轻微影响。应用定量转录组学方法进一步在转录水平上破译响应机制。
