Zhang Lilong, Chen Chen, Chai Dongqi, Kuang Tianrui, Deng Wenhong, Wang Weixing
Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.
Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.
Front Microbiol. 2023 Jan 6;13:1090392. doi: 10.3389/fmicb.2022.1090392. eCollection 2022.
Intrahepatic cholangiocarcinoma (ICC) is a silent liver malignancy with an increasing incidence. Gut mycobiota plays a crucial role in benign liver diseases; however, its correlation with ICC remains elusive. This study aimed to elucidate fungal differences in patients with ICC compared to healthy controls.
The 40 fecal samples from 23 ICC patients and 17 healthy controls were collected and analyzed using ITS2 rDNA sequencing. Obtaining the OTUs and combining effective grouping, we carried out the biodiversity and composition of the fungi, as well as FUNGuild functional annotation.
Our results revealed the presence of intestinal fungal dysbiosis with significant enrichment of opportunistic pathogenic fungi such as and and significant depletion of the beneficial fungus in ICC patients compared with healthy controls. Alpha-diversity analysis demonstrated that patients with ICC showed decreased fungal diversity compared to healthy controls. Beta diversity analysis indicated that the two groups exhibited significant segregated clustering. Besides, was found to be significantly more abundant in the ICC patients with TNM stage III-IV than those with stage I-II. The FUNGuild functional classification predicted that pathotrophs were the most abundant taxon in the ICC group, well above their abundance in healthy controls.
This study indicates that dysbiosis of the fecal mycobiome might be involved in ICC development. Further research into gut fungi may contribute to new therapeutic options for ICC patients.
肝内胆管癌（ICC）是一种隐匿性肝脏恶性肿瘤，发病率呈上升趋势。肠道微生物群在良性肝脏疾病中起关键作用；然而，其与ICC的相关性仍不明确。本研究旨在阐明ICC患者与健康对照者之间的真菌差异。
收集23例ICC患者和17例健康对照者的40份粪便样本，采用ITS2 rDNA测序进行分析。获得操作分类单元（OTUs）并结合有效分组，我们对真菌的生物多样性、组成以及FUNGuild功能注释进行了分析。
我们的结果显示，与健康对照者相比，ICC患者存在肠道真菌生态失调，机会性致病真菌如 和 显著富集，有益真菌 显著减少。α多样性分析表明，与健康对照者相比，ICC患者的真菌多样性降低。β多样性分析表明，两组表现出明显的分离聚类。此外，发现TNM III-IV期ICC患者中的 比I-II期患者明显更丰富。FUNGuild功能分类预测，致病营养型是ICC组中最丰富的分类群，远高于其在健康对照者中的丰度。
本研究表明，粪便真菌微生物群失调可能与ICC的发生有关。对肠道真菌的进一步研究可能有助于为ICC患者提供新的治疗选择。
