Wang Lamei, Cheng Yuxin, Hu Xiaoxia, Huang Yongguang
Key Laboratory of Fermentation Engineering and Biological Pharmacy of Guizhou Province, College of Liquor and Food Engineering, Guizhou University, Guiyang, Guizhou, China.
Guizhou Moutai Brewery (Group) Xijiu Co., Ltd., Xishui, Guizhou, China.
Front Nutr. 2023 Jan 4;9:1078132. doi: 10.3389/fnut.2022.1078132. eCollection 2022.
High-temperature Daqu is an important saccharifying fermenting starter for brewing Jiang-flavored Baijiu. This paper analyzed the diversity characteristics of bacterial communities of Jiang-flavored Daqu (JFDQ) with seasonal changes through Illumina HiSeq sequencing and multivariate statistical methods. Results showed that 21 phyla, 529 genera, and 47 core bacterial genera were identified from the 48 composite samples. Among them, eight functional genera were only found in the summer-produced Daqu (, etc.). , , , and , respectively, served as the representative functional bacterial genera of the four seasons. Functional prediction analysis showed that Amino acid metabolism Carbohydrate metabolism, Lipid metabolism, Metabolism of cofactors and vitamins, and Nucleotide metabolism (relative abundance > 1%) were the most critical microbial functions in JFDQ, and these key enzymes involved in acetoin biosynthesis, and acetyl-CoA biosynthesis were more abundant in the summer than in the winter. The functional microorganisms community in this paper would provide valuable suggestions about the seasonal production of JFDQ, guiding the Baijiu brewing processes.
高温大曲是酿造酱香型白酒的重要糖化发酵剂。本文通过Illumina HiSeq测序和多元统计方法分析了酱香型大曲（JFDQ）细菌群落随季节变化的多样性特征。结果表明，从48个复合样品中鉴定出21个门、529个属和47个核心细菌属。其中，8个功能属仅在夏季生产的大曲中发现（等）。 、 、 和 分别作为四季的代表性功能细菌属。功能预测分析表明，氨基酸代谢、碳水化合物代谢、脂质代谢、辅因子和维生素代谢以及核苷酸代谢（相对丰度>1%）是JFDQ中最关键的微生物功能，参与乙偶姻生物合成和乙酰辅酶A生物合成的这些关键酶在夏季比冬季更丰富。本文中的功能微生物群落将为JFDQ的季节性生产提供有价值的建议，指导白酒酿造工艺。
