Wang Yurong, Cai Wenchao, Wang Wenping, Shu Na, Zhang Zhendong, Hou Qiangchuan, Shan Chunhui, Guo Zhuang
Hubei Provincial Engineering and Technology Research Center for Food Ingredients Hubei University of Arts and Science Xiangyang China.
School of Food Science Shihezi University Shihezi China.
Food Sci Nutr. 2020 Dec 17;9(2):1003-1016. doi: 10.1002/fsn3.2068. eCollection 2021 Feb.
Bacterial communities that enrich in high-temperature Daqu are important for the Chinese maotai-flavor liquor brewing process. However, the bacterial communities in three different types of high-temperature Daqu (white Daqu, black Daqu, and yellow Daqu) are still undercharacterized. In this study, the bacterial diversity of three different types of high-temperature Daqu was investigated using Illumina MiSeq high-throughput sequencing. The bacterial community of high-temperature Daqu is mainly composed of thermophilic bacteria, and seven bacterial phyla along with 262 bacterial genera were identified in all 30 high-temperature Daqu samples. Firmicutes, Actinobacteria, Proteobacteria, and Acidobacteria were the dominant bacterial phyla in high-temperature Daqu samples, while , , , , , , and were the dominant bacterial genera. The bacterial community structure of three different types of high-temperature Daqu was significantly different ( < .05). In addition, the results of microbiome phenotype prediction by BugBase and bacterial functional potential prediction using PICRUSt show that bacteria from different types of high-temperature Daqu have similar functions as well as phenotypes, and bacteria in high-temperature Daqu have vigorous metabolism in the transport and decomposition of amino acids and carbohydrates. These results offer a reference for the comprehensive understanding of bacterial diversity of high-temperature Daqu.
在高温大曲中富集的细菌群落对中国酱香型白酒酿造过程至关重要。然而，三种不同类型的高温大曲（白曲、黑曲和黄曲）中的细菌群落仍未得到充分表征。在本研究中，使用Illumina MiSeq高通量测序技术对三种不同类型的高温大曲的细菌多样性进行了调查。高温大曲的细菌群落主要由嗜热细菌组成，在所有30个高温大曲样品中鉴定出了7个细菌门以及262个细菌属。厚壁菌门、放线菌门、变形菌门和酸杆菌门是高温大曲样品中的优势细菌门，而 、 、 、 、 、 、 和 是优势细菌属。三种不同类型的高温大曲的细菌群落结构存在显著差异（ < 0.05）。此外，通过BugBase进行的微生物组表型预测和使用PICRUSt进行的细菌功能潜力预测结果表明，来自不同类型高温大曲的细菌具有相似的功能和表型，并且高温大曲中的细菌在氨基酸和碳水化合物的运输和分解方面具有旺盛的代谢。这些结果为全面了解高温大曲的细菌多样性提供了参考。
