Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD, United States.
Laboratory of Microbial Processes in Foods, Department of Food Engineering, Federal University of Paraíba, João Pessoa, Brazil.
Lett Appl Microbiol. 2023 Jan 23;76(1). doi: 10.1093/lambio/ovac045.
Salmonella enterica subsp. enterica serovar Enteritidis (SE) has become the prevalent serovar isolated from gastroenteritis cases in Brazil since the 1990s. To better understand the genomic diversity and phylogenetic relationship amongst SE epidemic isolates from Brazil, 30 SE isolates from a variety of implicated foods and case patients of outbreaks between 1999 and 2006 were selected for genome comparison analyses. SE genomes were also compared against publicly available Brazilian SE isolates from pre- and postepidemic period. MLST analysis revealed that all isolates belong to sequence type (ST) 11. A total of seven Salmonella pathogenicity islands (SPIs) (SPI-1, SPI-3-5, SPI-13, SPI14, and C63PI) were identified in the evaluated genomes and all studied SE genomes carried similar prophage profiling. Resistome analysis revealed the presence of resistance genes to aminoglycosides [aac(6')laa, aph(3")-lb, aph(6)-ld], as well as point mutations in gyrA. Phylogenetic analysis demonstrated that certain isolates have circulated in Brazil for years and been involved in distinct outbreaks.
自 20 世纪 90 年代以来，肠炎沙门氏菌亚种肠炎血清型（SE）已成为巴西肠胃炎病例中分离出的主要血清型。为了更好地了解巴西 SE 流行分离株的基因组多样性和系统发育关系，从 1999 年至 2006 年各种相关食品和暴发病例中选择了 30 株 SE 分离株进行基因组比较分析。还将 SE 基因组与流行前和流行后的巴西公开可用的 SE 分离株进行了比较。多位点序列分型（MLST）分析显示，所有分离株均属于序列型（ST）11。在所评估的基因组中鉴定出了总共 7 个沙门氏菌致病岛（SPI）（SPI-1、SPI-3-5、SPI-13、SPI14 和 C63PI），所有研究的 SE 基因组都具有相似的噬菌体谱。耐药组分析显示存在对氨基糖苷类药物（aac(6')laa、aph(3")-lb、aph(6)-ld）的耐药基因，以及在 gyraA 中存在点突变。系统发育分析表明，某些分离株在巴西已经流传多年，并参与了不同的暴发。
