Price C A, Morris B A, O'Shea T, Webb R
Department of Reproduction and Growth Physiology, A.F.R.C. Institute of Animal Physiology, Roslin, Midlothian, U.K.
J Reprod Fertil. 1987 Sep;81(1):161-8. doi: 10.1530/jrf.0.0810161.
Yearling cross-bred heifers were injected with 0.4 or 4 mg total protein in non-ulcerative Freund's adjuvant (Groups A and C, respectively; N = 5 per group), or adjuvant with C. parvum (Groups B and D, respectively; N = 5 per group). Control heifers were not treated (N = 9). No antibodies were detected in heifers in Groups A and C and so these were excluded from the analysis. There was no effect of the treatment on Group B or D heifers after the priming or first booster injection. After a second booster injection one Group D heifer gave a triple ovulation, and two Group D heifers and one Group B heifer gave double ovulations, but for one oestrous cycle only. Group B heifers also showed a significant rise in FSH concentrations after the second booster injection. Group D heifers were given a third booster injection; there was an increase in the mean number of large follicles per heifer, and a decrease in oestrous cycle length, but no increase in ovulation rate. These results indicate that a transient increase in ovulation rate can be induced by actively immunizing cattle against partly purified follicular fluid from sheep.
将一岁的杂交小母牛分别用0.4毫克或4毫克总蛋白与非溃疡性弗氏佐剂混合注射（分别为A组和C组，每组n = 5），或与微小隐孢子虫佐剂混合注射（分别为B组和D组，每组n = 5）。对照小母牛不进行处理（n = 9）。在A组和C组的小母牛中未检测到抗体，因此将这些小母牛排除在分析之外。在初次免疫或首次加强注射后，处理对B组或D组小母牛没有影响。在第二次加强注射后，1头D组小母牛出现了三联排卵，2头D组小母牛和1头B组小母牛出现了双排卵，但仅持续了一个发情周期。第二次加强注射后，B组小母牛的促卵泡激素浓度也显著升高。对D组小母牛进行了第三次加强注射；每头小母牛的大卵泡平均数量增加，发情周期长度缩短，但排卵率没有增加。这些结果表明，通过主动免疫牛使其针对部分纯化的绵羊卵泡液，可以诱导排卵率短暂升高。
