Morris D G, McDermott M G, Diskin M G, Morrison C A, Swift P J, Sreenan J M
Animal Reproduction Department, Agriculture and Food Development Authority, Belclare, Tuam, Co. Galway, Ireland.
J Reprod Fertil. 1993 Jan;97(1):255-61. doi: 10.1530/jrf.0.0970255.
Three peptide sequences from the bovine inhibin alpha-subunit (P1: 18-30; P2: 63-72 and P3: 107-122) were synthesized and conjugated to human serum albumin (HSA). Hereford cross-heifers (n = 5 per group) were injected with 3 mg of one of the peptide conjugates, followed by three booster injections at intervals of 11 weeks. Control heifers (n = 5) were injected with HSA only. Antibodies recognizing both the individual peptides and 32 kDa bovine inhibin were generated and ovulation rate was increased in peptide immunized heifers. In group P1, 1 of 5 heifers responded with an increased ovulation rate whereas in groups P2 and P3, 5 of 5 and 4 of 5 heifers, respectively, had an increased ovulation rate. In group P2, in the first oestrous cycle following booster injections 2 and 3, 4 of 5 and 3 of 5 heifers, respectively, responded with twin ovulations, whereas a fourth heifer had three ovulations following booster injection 3. After breeding following booster injection 3, 3 of 5 heifers in group P2 and 1 of 5 in group P3 gave birth to twin calves. This study demonstrates the potential of immunizing against synthetic peptide sequences of the alpha-subunit of bovine inhibin to increase ovulation and twinning rates in cattle.
合成了来自牛抑制素α亚基的三个肽序列（P1：18 - 30；P2：63 - 72和P3：107 - 122），并将其与人血清白蛋白（HSA）偶联。每组5头海福特杂交小母牛分别注射3毫克其中一种肽偶联物，之后每隔11周进行三次加强注射。对照小母牛（n = 5）仅注射HSA。产生了识别单个肽和32 kDa牛抑制素的抗体，且肽免疫的小母牛排卵率增加。在P1组中，5头小母牛中有1头排卵率增加；而在P2组和P3组中，分别有5头中的5头和5头中的4头排卵率增加。在P2组中，在加强注射2和3后的第一个发情周期，分别有5头中的4头和5头中的3头出现双排卵，而第四头小母牛在加强注射3后出现三次排卵。在加强注射3后配种，P2组5头小母牛中有3头、P3组5头中有1头产下双胞胎犊牛。本研究证明了针对牛抑制素α亚基的合成肽序列进行免疫接种以提高牛的排卵率和双胎率的潜力。
