Jaw1/LRMP与高尔基体带状结构的维持有关。
Jaw1/LRMP is associated with the maintenance of Golgi ribbon structure.
作者信息
Okumura Wataru, Tadahira Kazuko, Kozono Takuma, Tamura-Nakano Miwa, Sato Hiroyuki, Matsui Hitomi, Dohi Taeko, Rohrer Jack, Tonozuka Takashi, Nishikawa Atsushi
机构信息
Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Communal Laboratory, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.
出版信息
J Biochem. 2023 Apr 26;173(5):383-392. doi: 10.1093/jb/mvad004.
Jaw1/LRMP is a membrane protein that is localized to the endoplasmic reticulum and outer nuclear membrane. Previously, we revealed that Jaw1 functions to maintain nuclear shape by interacting with microtubules as a Klarsicht/ANC-1/Syne/homology (KASH) protein. The loss of several KASH proteins causes defects in the position and shape of the Golgi apparatus as well as the nucleus, but the effects of Jaw1 depletion on the Golgi apparatus were poorly understood. Here, we found that siRNA-mediated Jaw1 depletion causes Golgi fragmentation with disordered ribbon structure in the melanoma cell, accompanied by the change in the localization of the Golgi-derived microtubule network. Thus, we suggest that Jaw1 is a novel protein to maintain the Golgi ribbon structure, associated with the microtubule network.
Jaw1/LRMP是一种定位于内质网和外核膜的膜蛋白。此前，我们发现Jaw1作为一种Klarsicht/ANC-1/Syne/同源性（KASH）蛋白，通过与微管相互作用来维持核形态。几种KASH蛋白的缺失会导致高尔基体以及细胞核的位置和形态出现缺陷，但Jaw1缺失对高尔基体的影响却知之甚少。在这里，我们发现小干扰RNA（siRNA）介导的Jaw1缺失会导致黑色素瘤细胞中高尔基体碎片化，其带状结构紊乱，并伴有高尔基体衍生的微管网络定位的改变。因此，我们认为Jaw1是一种与微管网络相关的、维持高尔基体带状结构的新蛋白。
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