Paulo Picanço School of Dentistry, Fortaleza, Brazil.
Vitreous Materials Laboratory (LAMAV), Department of Materials Engineering, Federal University of São Carlos, São Carlos, Brazil.
Dent Mater. 2023 Feb;39(2):217-226. doi: 10.1016/j.dental.2023.01.005. Epub 2023 Jan 21.
To analyze simplified adhesive containing pure or silanized bioglass 45S5 (with calcium) or Sr-45S5 (strontium-substituted) fillers applied on dentin and to evaluate the microtensile bond strength (µTBS), interface nanoleakage, degree of conversion of adhesive, collagen degradation and remineralization.
Ambar Universal adhesive (FGM) was doped with 10 wt% bioactive glasses to form following groups: Control (no bioglass), 45S5 (conventional bioglass 45S5), Sr-45S5 (Sr-substituted bioglass 45S5), Sil-45S5 (silanized bioglass 45S5) and Sil-Sr-45S5 (silanized bioglass Sr-45S5). Adhesives were applied after dentin acid-etching using phosphoric acid at extracted human molars. Resin-dentin sticks were obtained and tested for µTBS, nanoleakage at 24 h or 6 months. Degree of conversion was measured using micro-Raman spectroscopy. Dentin remineralization was assessed by FTIR after 6-month storage in PBS. Hydroxyproline (HYP) release was surveyed by UV-Vis spectroscopy. Statistical analysis was performed using ANOVA and Tukey's test (p < 0.05).
Regarding µTBS, Sr-45S5 and 45S5 presented higher and stable results (p > 0.05). Control (p = 0.018) and Sil-Sr-45S5 (p < 0.001) showed µTBS reduction after 6-month aging. Sil-Sr-45S5 showed higher HYP release than that obtained in the 45S5 group. Sil-45S5 showed mineral deposition and increase in µTBS (p = 0.028) after 6-months. All experimental adhesives exhibited higher degree of conversion compared to Control group, except for 45S5. All adhesives created gap-free interfaces, with very low silver impregnation, except for Sil-Sr-45S5.
The incorporation of silanized 45S5 bioglass into the universal adhesive was advantageous in terms of dentin remineralization, bonding performance and adhesive polymerization. Conversely, Sil-Sr-45S5 compromised the µTBS, interface nanoleakage and had a negative impact on HYP outcomes.
分析应用于牙本质的含有纯或硅烷化生物玻璃 45S5（含钙）或 Sr-45S5（锶取代）填料的简化胶粘剂，并评估微拉伸粘结强度（µTBS）、界面纳米渗漏、粘结剂的转化率、胶原降解和再矿化。
Ambar 通用胶粘剂（FGM）中掺杂了 10wt%生物活性玻璃，形成以下组：对照（不含生物玻璃）、45S5（传统生物玻璃 45S5）、Sr-45S5（锶取代生物玻璃 45S5）、Sil-45S5（硅烷化生物玻璃 45S5）和 Sil-Sr-45S5（硅烷化生物玻璃 Sr-45S5）。在提取的人磨牙上用磷酸酸蚀牙本质后，应用胶粘剂。获得树脂-牙本质棒，并测试µTBS、24 小时或 6 个月的纳米渗漏。使用微拉曼光谱测量转化率。在 PBS 中储存 6 个月后，通过 FTIR 评估牙本质再矿化。通过 UV-Vis 光谱测量羟脯氨酸（HYP）释放。使用方差分析和 Tukey 检验（p<0.05）进行统计分析。
关于µTBS，Sr-45S5 和 45S5 呈现出更高和更稳定的结果（p>0.05）。对照组（p=0.018）和 Sil-Sr-45S5（p<0.001）在 6 个月老化后显示µTBS 降低。Sil-Sr-45S5 释放的 HYP 高于 45S5 组。Sil-45S5 在 6 个月后表现出矿物质沉积和 µTBS 增加（p=0.028）。与对照组相比，所有实验性胶粘剂的转化率均高于对照组，除 45S5 外。所有的胶粘剂都形成了无间隙的界面，只有很少的银渗透，除了 Sil-Sr-45S5。
将硅烷化 45S5 生物玻璃掺入通用胶粘剂中有利于牙本质再矿化、粘结性能和胶粘剂聚合。相反，Sil-Sr-45S5 降低了 µTBS、界面纳米渗漏，并对 HYP 结果产生负面影响。
