Branger C, Goullet P
Laboratoire de Microbiologie, Faculté de Médecine Xavier-Bichat, Université Paris VII, France.
J Med Microbiol. 1987 Nov;24(3):275-81. doi: 10.1099/00222615-24-3-275.
Methicillin-sensitive and methicillin-resistant strains of Staphylococcus aureus from diverse geographic origins were analysed by polyacrylamide-agarose gel electrophoresis for esterase polymorphism. Three kinds of esterase bands, designated A, B and C, were defined by their ranges of activity toward five synthetic substrates and their resistance to di-isopropyl fluorophosphate. There were five allozymes of esterase A, four of esterase B and four of esterase C. Eighteen distinct combinations of allozymes (zymotypes) were distinguished amongst 105 strains analysed. Two major zymotypes were represented by 35 and 19 strains respectively, whereas other zymotypes were represented by one or, at most, seven strains. The coefficient of genetic diversity was lower for methicillin-resistant strains than for methicillin-sensitive strains. Most of the methicillin-resistant strains are represented by the two major zymotypes which differed from each other by the electrophoretic behaviour of the three esterases. These results indicate that, on the basis of esterase electrophoretic polymorphism, methicillin resistance is expressed in genetically different strains.
采用聚丙烯酰胺 - 琼脂糖凝胶电泳法，对来自不同地理区域的甲氧西林敏感和耐甲氧西林金黄色葡萄球菌菌株进行酯酶多态性分析。根据三种酯酶对五种合成底物的活性范围及其对二异丙基氟磷酸的抗性，定义了三种酯酶带，分别为A、B和C。酯酶A有五种同工酶，酯酶B有四种，酯酶C有四种。在所分析的105株菌株中，区分出了18种不同的同工酶组合（酶型）。两种主要酶型分别由35株和19株菌株代表，而其他酶型仅由1株或最多7株菌株代表。耐甲氧西林菌株的遗传多样性系数低于甲氧西林敏感菌株。大多数耐甲氧西林菌株由两种主要酶型代表，这两种酶型在三种酯酶的电泳行为上彼此不同。这些结果表明，基于酯酶电泳多态性，甲氧西林抗性在基因不同的菌株中表现出来。
