Rasouli Ghahroudi Amir Alireza, Rokn Amir Reza, Kalhori Katayoun A M, Khorsand Afshin, Pournabi Alireza, Pinheiro A L B, Fekrazad Reza
Periodontology Department, Tehran University of Medical Sciences, Tehran, Iran,
Lasers Med Sci. 2014 May;29(3):925-32. doi: 10.1007/s10103-013-1403-5. Epub 2013 Aug 31.
This study aims to assess the effect of low-level laser therapy (LLLT) irradiation and Bio-Oss graft material on the osteogenesis process in the rabbit calvarium defects. Twelve white male New Zealand rabbits were included in this study. Four 8-mm diameter identical defects were prepared on each rabbit's calvarium. One site was left as an untreated control (C), the second site was filled with Bio-Oss (B), the third site was treated with laser irradiation (L), and the fourth site treated with Bio-Oss and laser irradiation (B + L). In the laser group, a diode laser (wavelength 810 nm, output power 300 mW, irradiation mode CW, energy density 4 J/cm2) was applied immediately after surgery and then one other day for the next 20 days. After 4 and 8 weeks, the animals were sacrificed and histological and histomorphometric examinations were performed and the data were subjected to Friedman and repeated measurements ANOVA tests. Significant differences were not found regarding inflammation severity, foreign body reactions, and vitality of newly formed bone on 4th and 8th week after operation. The mean amount of new bone was 15.83 and 18.5% in the controls on the 4th and 8th week; 27.66 and 25.16% in the laser-irradiated group; 35.0 and 41.83% in Bio-Oss and 41.83 and 47.0% in the laser + Bio-Oss treated specimens with significant statistical differences (p <0.05). Application of LLLT in combination with Bio-Oss® can promote bone healing. Therefore, LLLT may be clinically beneficial in promoting bone formation in skeletal defects.
本研究旨在评估低强度激光疗法（LLLT）照射和Bio-Oss骨粉材料对兔颅骨缺损骨生成过程的影响。本研究纳入了12只白色雄性新西兰兔。在每只兔的颅骨上制备4个直径8毫米的相同缺损。一个部位留作未处理对照（C），第二个部位填充Bio-Oss骨粉（B），第三个部位接受激光照射（L），第四个部位接受Bio-Oss骨粉和激光照射（B + L）。在激光组中，术后立即应用二极管激光（波长810 nm，输出功率300 mW，照射模式连续波，能量密度4 J/cm²），然后在接下来的20天里每隔一天照射一次。在4周和8周后，处死动物并进行组织学和组织形态计量学检查，数据进行Friedman检验和重复测量方差分析。术后第4周和第8周，在炎症严重程度、异物反应和新形成骨的活力方面未发现显著差异。对照组在第4周和第8周新骨的平均量分别为15.83%和18.5%；激光照射组为27.66%和25.16%；Bio-Oss骨粉组为35.0%和41.83%；激光+Bio-Oss骨粉处理组为41.83%和47.0%，差异有统计学意义（p <0.05）。低强度激光疗法与Bio-Oss®联合应用可促进骨愈合。因此，低强度激光疗法在促进骨骼缺损的骨形成方面可能具有临床益处。
