Department of Clinical Microbiology, Lillebaelt Hospital, Vejle, University Hospital of Southern Denmark, Vejle, Denmark.
Department of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark.
Eur J Clin Microbiol Infect Dis. 2023 Mar;42(3):277-285. doi: 10.1007/s10096-023-04553-5. Epub 2023 Jan 24.
The purpose of this study was to examine the transfer rate of SARS-CoV-2 IgG antibodies in pregnancy and newborns. Two Danish labor wards screened all women for SARS-CoV-2 by PCR upon arrival. Women (n = 99) with a SARS-CoV-2 PCR-positive nasopharyngeal (NP) swab or with a household member with a positive swab at labor or any time during pregnancy, or COVID-19 symptoms upon admission (November 2020 through August 2021), were included. Mother and infant were tested by NP swabs at delivery, and maternal and infant (umbilical cord) venous blood samples were collected. We obtained clinical information including previous PCR test results from the medical records. SARS-Cov-2 IgM and quantified IgG antibodies were measured by enzyme-linked immunosorbent assay and transfer ratios of IgG. We detected IgG antibodies in 73 women and 65 cord blood sera and found a strong correlation between SARS-CoV-2 IgG concentrations in maternal and umbilical cord sera (r = 0.9; p < 0.05). Transfer ratio was > 1.0 in 51 out of 73 (69%) infants and > 1.5 in 26 (35%). We found that transfer was proportional to time from a positive SARS-CoV-2 PCR NP swab to delivery (r = 0.5; p < 0.05). Transfer ratios of SARS-CoV-2 antibodies were associated with time from infection to delivery with transfer ratios of more than 1.0 in the majority of seropositive mother-infant dyads.
这项研究的目的是检测 SARS-CoV-2 IgG 抗体在妊娠和新生儿中的转移率。丹麦的两个产房通过 PCR 对所有女性进行 SARS-CoV-2 筛查。在分娩时，对鼻咽 (NP) 拭子 SARS-CoV-2 PCR 阳性或有家庭成员在分娩时或怀孕期间任何时候 SARS-CoV-2 PCR 阳性或有 COVID-19 症状入院的女性 (2020 年 11 月至 2021 年 8 月) 及其婴儿进行 NP 拭子检测，同时采集母亲和婴儿 (脐带) 静脉血样。我们从病历中获得了包括之前 PCR 检测结果在内的临床信息。采用酶联免疫吸附试验检测 SARS-Cov-2 IgM 和定量 IgG 抗体，并测定 IgG 抗体的转移率。我们在 73 名产妇和 65 名脐带血血清中检测到 IgG 抗体，发现母体和脐带血清中 SARS-CoV-2 IgG 浓度之间存在很强的相关性 (r=0.9；p<0.05)。在 73 名婴儿中，有 51 名 (69%) 的转移比值 >1.0，26 名 (35%) 的转移比值 >1.5。我们发现，转移与从 SARS-CoV-2 PCR 阳性 NP 拭子到分娩的时间成正比 (r=0.5；p<0.05)。SARS-CoV-2 抗体的转移比值与从感染到分娩的时间有关，大多数 SARS-CoV-2 抗体阳性的母婴对的转移比值 >1.0。
