Promsuwan Kiattisak, Soleh Asamee, Samoson Kritsada, Saisahas Kasrin, Wangchuk Sangay, Saichanapan Jenjira, Kanatharana Proespichaya, Thavarungkul Panote, Limbut Warakorn
Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Forensic Science Innovation and Service Center, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
Talanta. 2023 May 1;256:124266. doi: 10.1016/j.talanta.2023.124266. Epub 2023 Jan 11.
Near-field communication (NFC) was used to control a portable glucose biosensor for diabetes diagnosis. The system comprised a smartphone and an NFC potentiostat connected to a screen-printed carbon electrode (SPCE) modified with Prussian blue-graphene ink and functionalized with gold nanoparticles-embedded poly (3,4ethylene dioxythiophene):polysulfonic acid coated with glucose oxidase (GOx-AuNPs-PEDOT:PSS/PB-G). GOx catalyzed the glucose redox reaction while the conductivity and sensitivity of the AuNPs-PEDOT:PSS composite enhanced electron transfer to the PB-G, which was used as a mediator. The fabrication process was characterized by scanning electron microscopy (SEM) with energy dispersibe x-ray analysis (EDX). The platform was electrochemically characterized by electrical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The NFC biosensing device was then applied to quantify glucose in human blood serum by amperometry. The linear concentration range and detection limit for glucose were 0.5-500 μM and 0.15 μM, respectively. The accuracy of the device was good and results were in agreement with the results obtained from the standard hospital method. This NFC glucose sensing device can be a simple, sensitive, selective and portable platform for medical diagnosis.
近场通信（NFC）被用于控制一种用于糖尿病诊断的便携式葡萄糖生物传感器。该系统由一部智能手机和一个NFC恒电位仪组成，NFC恒电位仪连接到一个用普鲁士蓝-石墨烯墨水修饰、并用嵌入金纳米颗粒的聚（3,4-亚乙基二氧噻吩）：聚磺酸（涂有葡萄糖氧化酶，即GOx-AuNPs-PEDOT:PSS/PB-G）功能化的丝网印刷碳电极（SPCE）。葡萄糖氧化酶催化葡萄糖氧化还原反应，而金纳米颗粒-聚（3,4-亚乙基二氧噻吩）：聚磺酸复合材料的导电性和灵敏度增强了向用作媒介物的普鲁士蓝-石墨烯的电子转移。制造过程通过扫描电子显微镜（SEM）和能量色散X射线分析（EDX）进行表征。该平台通过电化学阻抗谱（EIS）和循环伏安法（CV）进行电化学表征。然后，NFC生物传感装置通过安培法用于定量测定人血清中的葡萄糖。葡萄糖的线性浓度范围和检测限分别为0.5 - 500 μM和0.15 μM。该装置的准确性良好，结果与标准医院方法获得的结果一致。这种NFC葡萄糖传感装置可以成为一种用于医学诊断的简单、灵敏、选择性好且便携的平台。
