State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
Pharmacognosy Department, College of Pharmacy, Cairo University, Kasr El Aini St., P.B. 11562 Cairo, Egypt.
J Adv Res. 2023 Oct;52:73-88. doi: 10.1016/j.jare.2023.01.013. Epub 2023 Jan 21.
Dietary fiber and flavonoids are promising drugs reported in the treatment of inflammatory bowel disease (IBD). However, it is unclear the interaction between dietary fiber and flavonoids in gut health.
The therapeutic effect of celery, kale, and red chicory powders on colitis mice using non-group feeding cages was investigated. Further, the efficacy of whole celery, celery soluble dietary fiber (CSDF), celery insoluble dietary fiber (CIDF), celery flavonoids (CF), CSDF + CF and CIDF + CF in IBD mice model was assessed to dissect protective effect to attribute to which component(s) in such complex matrix.
3% Dextran sulfate sodium salt (DSS) was used to induce mice colitis model. Multiple molecular biological methods were employed to evaluate the severity of mice colitis and the gut microbial composition of mice.
Administration of kale and red chicory significantly restored body weight, DAI score, and colon length in colonic mice, and celery showed the weakest effects. Administration of either CSDF or CF markedly improved the histological damage, increased colonic mucus expression, and reduced colonic MPO/iNOS activities, and IL-6/IL-1β levels. However, CSDF + CF showed weaker improvement than CF or SDF in most physical and biochemical signs. Furthermore, CSDF and CF decreased intestinal g_Escherichia-Shihella and g_Clostridium_sensu_stricto_1 induced by DSS administration. Interestingly, celery flavonoid promoted g_Akkermansia proliferation both in vivo and in vitro, and which can be inhibited by CSDF.
This study revealed for the first time that CSDF can suppress the protective effect of CF on intestinal health by inhibiting g_Akkermansia, and clarified that the decreased efficacy of celery whole food on colitis was mediated by an antagonism between CSDF and CF. Moreover, this study presents for the first time that interaction between soluble dietary fiber and flavonoids in vivo can ameliorate the efficacy of dietary fiber or flavonoids when administered alone suggestive for an antagonistic effect.
膳食纤维和类黄酮是治疗炎症性肠病（IBD）的有前途的药物。然而，膳食纤维和类黄酮在肠道健康中的相互作用尚不清楚。
用非分组饲养笼研究芹菜、羽衣甘蓝和红菊苣粉对结肠炎小鼠的治疗作用。进一步评估整芹菜、芹菜可溶性膳食纤维（CSDF）、芹菜不溶性膳食纤维（CIDF）、芹菜类黄酮（CF）、CSDF+CF 和 CIDF+CF 在 IBD 小鼠模型中的疗效，以剖析保护作用归因于这种复杂基质中的哪个（些）成分。
用 3%葡聚糖硫酸钠（DSS）诱导小鼠结肠炎模型。采用多种分子生物学方法评价小鼠结肠炎的严重程度和肠道微生物组成。
羽衣甘蓝和红菊苣的给药显著恢复了结肠小鼠的体重、DAI 评分和结肠长度，而芹菜的作用最弱。CSDF 或 CF 的给药显著改善了组织学损伤，增加了结肠粘液表达，并降低了结肠 MPO/iNOS 活性和 IL-6/IL-1β 水平。然而，CSDF+CF 在大多数生理和生化指标上的改善弱于 CF 或 SDF。此外，CSDF 和 CF 降低了 DSS 给药诱导的肠道 g_Escherichia-Shihella 和 g_Clostridium_sensu_stricto_1。有趣的是，芹菜类黄酮在体内和体外均促进 g_Akkermansia 的增殖，而 CSDF 可抑制其增殖。
本研究首次揭示 CSDF 可通过抑制 g_Akkermansia 抑制 CF 对肠道健康的保护作用，并阐明整芹菜食物对结肠炎疗效降低是由 CSDF 和 CF 之间的拮抗作用介导的。此外，本研究首次表明，体内可溶性膳食纤维和类黄酮之间的相互作用可改善膳食纤维或类黄酮单独给药时的疗效，提示存在拮抗作用。
