Xiong Shijie, Jiang Jiajia, Wan Fan, Tan Ding, Zheng Haibo, Xue Huiqin, Hang Yiqiong, Lu Yang, Su Yong
Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Antioxidants (Basel). 2024 Apr 8;13(4):441. doi: 10.3390/antiox13040441.
Cordycepin is considered a major bioactive component in extract. This study was performed to evaluate the ameliorative effect of extract (CME) and cordycepin (CPN) supplementation on intestinal damage in LPS-challenged piglets. The results showed that CPN or CME supplementation significantly increased the villus height ( < 0.01) and villus height/crypt depth ratio ( < 0.05) in the jejunum and ileum of piglets with LPS-induced intestinal inflammation. Meanwhile, CPN or CME supplementation alleviated oxidative stress and inflammatory responses by reducing the levels of MDA ( < 0.05) and pro-inflammatory cytokines in the serum. Additionally, supplementation with CPN or CME modulated the structure of the intestinal microbiota by enriching short-chain fatty acid-producing bacteria, and increased the level of butyrate ( < 0.05). The RNA-seq results demonstrated that CME or CPN altered the complement and coagulation-cascade-related genes ( < 0.05), including upregulating gene while downregulating the genes , , , , , , , and , which regulate the complement activation involved in inflammatory and immune responses. Correlation analysis further demonstrated the potential relation between the gut microbiota and intestinal inflammation, oxidative stress, and butyrate in piglets. In conclusion, CPN or CME supplementation might inhibit LPS-induced inflammation and oxidative stress by modulating the intestinal microbiota and its metabolite butyrate in piglets.
虫草素被认为是提取物中的主要生物活性成分。本研究旨在评估提取物（CME）和补充虫草素（CPN）对脂多糖（LPS）攻击的仔猪肠道损伤的改善作用。结果表明，补充CPN或CME可显著增加LPS诱导肠道炎症仔猪空肠和回肠的绒毛高度（P<0.01）和绒毛高度/隐窝深度比值（P<0.05）。同时，补充CPN或CME可通过降低血清中丙二醛（MDA）水平（P<0.05）和促炎细胞因子来减轻氧化应激和炎症反应。此外，补充CPN或CME可通过富集产生短链脂肪酸的细菌来调节肠道微生物群结构，并增加丁酸盐水平（P<0.05）。RNA测序结果表明，CME或CPN改变了补体和凝血级联相关基因（P<0.05），包括上调基因 ，同时下调基因 、 、 、 、 、 、 和 ，这些基因调节参与炎症和免疫反应的补体激活。相关性分析进一步证明了仔猪肠道微生物群与肠道炎症、氧化应激和丁酸盐之间的潜在关系。总之，补充CPN或CME可能通过调节仔猪肠道微生物群及其代谢产物丁酸盐来抑制LPS诱导的炎症和氧化应激。
