Department of Biology, College of Science, Shantou University, Shantou 515063, Guangdong, PR China; Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, Guangdong, PR China.
Department of Biology, College of Science, Shantou University, Shantou 515063, Guangdong, PR China; Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, Guangdong, PR China.
J Biosci Bioeng. 2023 Mar;135(3):232-237. doi: 10.1016/j.jbiosc.2022.12.013. Epub 2023 Jan 22.
The microparticle-enhanced cultivation (MPEC) was used to enhance the production of Antrodin C by submerged fermentation of medicinal mushroom Antrodia cinnamomea. The crucial factors such as types, sizes, concentrations, and addition time of microparticles were optimized. The mechanism of MPEC on the membrane permeability and fluidity of A. cinnamomea and the expression of key genes in Antrodin C were investigated. When talc (18 μm, 2 g/L) was added into the fermentation liquid at 0 h, the promoting effect on Antrodin C was the best. The maximum yield of Antrodin C was 1615.7 mg/L, which was about 2.98 times of the control (541.7 mg/L). Talc slightly damaged the mycelia of A. cinnamomea, increased the release of intracellular constituents, and enhanced the index of unsaturated fatty acid. In addition, the key genes (IDI, E2.3.3.10, HMGCR, atoB) that might play an important role in the synthesis of the triquine-type sesquiterpene Antrodin C, were upregulated. In conclusion, talc increased the permeability and fluidity of cell membrane, upregulated the key genes and improved the biosynthesis process to enhance the yield of Antrodin C in the submerged fermentation of A. cinnamomea.
微载体强化培养（MPEC）用于通过药用蘑菇密环菌的液体深层发酵来提高安络小皮伞素 C 的产量。优化了微载体的类型、大小、浓度和添加时间等关键因素。研究了 MPEC 对密环菌细胞膜通透性和流动性以及安络小皮伞素 C 关键基因表达的影响。当在 0 小时将滑石（18μm，2g/L）添加到发酵液中时，对安络小皮伞素 C 的促进作用最好。安络小皮伞素 C 的最大产量为 1615.7mg/L，约为对照（541.7mg/L）的 2.98 倍。滑石轻微破坏了密环菌的菌丝体，增加了细胞内成分的释放，并提高了不饱和脂肪酸指数。此外，上调了可能在三萜型倍半萜安络小皮伞素 C 的合成中起重要作用的关键基因（IDI、E2.3.3.10、HMGCR、atoB）。总之，滑石增加了细胞膜的通透性和流动性，上调了关键基因并改善了生物合成过程，从而提高了密环菌液体深层发酵中安络小皮伞素 C 的产量。
