牛心肌中（顶复门：肉孢子虫科）的微观与分子检测
Microscopic and Molecular Detection of (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle.
作者信息
Dalir Ghaffari Ali, Dalimi Abdolhossein, Pirestani Majid
机构信息
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
出版信息
Iran J Parasitol. 2022 Oct-Dec;17(4):535-542. doi: 10.18502/ijpa.v17i4.11281.
BACKGROUND
is a genus of coccidian protozoa that at least seven species of it can parasitize cattle. The global prevalence of is close to 100% in adult cattle. The main aim of this study was to identify the infection rate of spp. in heart of cattle in Tehran, Iran by microscopy and PCR-RFLP methods.
METHODS
Totally, 100 bovine heart samples were collected from the main slaughterhouse of Shahriar, Meysam slaughterhouse, west of Tehran in 2016. At first, heart samples were completely examined for the presence of sarcocystic macrocysts. Then, for microscopic examination, 50 g of each heart was digested in sterile condition using pepsin acid digestion method. Then, the species of the parasite were detected by PCR-RFLP technique and sequencing.
RESULTS
Overall, 97 of 100 of the heart muscle samples were infected with . All the samples were detected as through similar patterns in PCR-RFLP.
CONCLUSION
is the most common species in the heart of cattle slaughtered in Shahriar.
背景
是球虫纲原生动物的一个属，其中至少有七种可寄生于牛。在成年牛中，其全球感染率接近100%。本研究的主要目的是通过显微镜检查和PCR-RFLP方法确定伊朗德黑兰牛心脏中 属物种的感染率。
方法
2016年，从德黑兰西部的沙赫里亚尔主屠宰场、梅萨姆屠宰场共采集了100份牛心脏样本。首先，对心脏样本进行全面检查，以确定是否存在肉孢子虫大囊。然后，为进行显微镜检查，每份心脏取50克，在无菌条件下采用胃蛋白酶酸消化法进行消化。然后，通过PCR-RFLP技术和测序检测寄生虫的种类。
结果
总体而言，100份心肌样本中有97份感染了 。在PCR-RFLP中，所有样本通过相似模式被检测为 。
结论
是在沙赫里亚尔屠宰的牛心脏中最常见的物种。
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