Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of Korea.
Biosensors (Basel). 2024 Mar 15;14(3):146. doi: 10.3390/bios14030146.
The global challenges posed by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic have underscored the critical importance of innovative and efficient control systems for addressing future pandemics. The most effective way to control the pandemic is to rapidly suppress the spread of the virus through early detection using a rapid, accurate, and easy-to-use diagnostic platform. In biosensors that use bioprobes, the binding affinity of molecular recognition elements (MREs) is the primary factor determining the dynamic range of the sensing platform. Furthermore, the sensitivity relies mainly on bioprobe quality with sufficient functionality. This comprehensive review investigates aptamers and nanobodies recently developed as advanced MREs for SARS-CoV-2 diagnostic and therapeutic applications. These bioprobes might be integrated into organic bioelectronic materials and devices, with promising enhanced sensitivity and specificity. This review offers valuable insights into advancing biosensing technologies for infectious disease diagnosis and treatment using aptamers and nanobodies as new bioprobes.
严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 大流行带来的全球挑战突显了创新和高效控制系统对于应对未来大流行的至关重要性。控制大流行的最有效方法是通过使用快速、准确和易于使用的诊断平台进行早期检测，迅速抑制病毒的传播。在使用生物探针的生物传感器中，分子识别元件 (MRE) 的结合亲和力是决定传感平台动态范围的主要因素。此外，灵敏度主要依赖于具有足够功能的生物探针质量。本综述研究了最近开发的作为 SARS-CoV-2 诊断和治疗应用的先进 MRE 的适体和纳米抗体。这些生物探针可以集成到有机生物电子材料和设备中，具有增强的灵敏度和特异性的潜力。本综述为使用适体和纳米抗体作为新型生物探针在传染病诊断和治疗中推进生物传感技术提供了有价值的见解。
